Histone H3.3B Overexpression Lysate (Native)

Images

 
Western Blot: Histone H3.3B Overexpression Lysate (Adult Normal) [NBL1-11429] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H3.3B.
Western Blot: Histone H3.3B Overexpression Lysate (Adult Normal) [NBL1-11429] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for H3F3B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Histone H3.3B Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202257 and based on accession number NM_005324. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens H3 histone, family 3B (H3.3B) (H3F3B), mRNA.
Gene
H3F3B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
15.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Histone H3.3B

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Histone H3.3B Overexpression Lysate (Native)

  • H3 histone, family 3A
  • H3 histone, family 3B (H3.3B)
  • H3.3BH3.3A
  • H3F3
  • H3F3A
  • histone H3.3

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Histone H3.3B Lysate (NBL1-11429) (0)

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Bioinformatics

Gene Symbol H3F3B