Histone H3.3A Antibody

Immunocytochemistry/Immunofluorescence: Histone H3.3A Antibody [NBP1-33767] - Paraformaldehyde-fixed HeLa, using Histone H3. 3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

Western Blot: Histone H3.3A Antibody [NBP1-33767] - Sample (30 ug of whole cell lysate) A: IMR32 15% SDS PAGE; antibody diluted at 1:1000.

Western Blot: Histone H3.3A Antibody [NBP1-33767] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3A antibody (NBP1-33767) diluted at 1:500.

Product Discontinued

Histone H3.3A Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Histone H3.3A.3. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H3-3A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Histone H3.3A Antibody

• H3 histone, family 3A
• H3.3AH3F3H3F3B
• H3.3B
• histone H3.3
• MGC87782
• MGC87783

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: H3-3A
• Entrez:
  • 3020(Human)
  • 3021(Human)