Histone H3.3A Antibody

Images

 
Western Blot: Histone H3.3A Antibody [NBP1-33767] - Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Histone H3.3A Antibody [NBP1-33767] - Paraformaldehyde-fixed HeLa, using Histone H3. 3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha ...read more
Western Blot: Histone H3.3A Antibody [NBP1-33767] - Sample (30 ug of whole cell lysate) A: IMR32 15% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Histone H3.3A Antibody [NBP1-33767] - Analysis of paraformaldehyde-fixed A431, using Histone H3.3B (NBP1-33767) antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.08 mg/ml

Order Details

Histone H3.3A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Histone H3.3. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H3F3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (100%) and Xenopus Tropicalis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.08 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H3.3A Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • histone H3.3
  • MGC87782
  • MGC87783

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol H3F3A
Entrez