Histone H3.3 Antibody

Images

 
Western Blot: Histone H3.3 Antibody [NBP2-14981] - A. 30 ug PC-12 whole cell lysate/extract 15 % SDS-PAGEHistone H3.3 antibody dilution: 1:10000
Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-14981] - Sample: Paraffin-embedded mouse middle brain. Histone H3.3 antibody dilution: 1:500.
Western Blot: Histone H3.3 Antibody [NBP2-14981] - A. 30 ug Jurkat whole cell lysate/extractB. 30 ug Raji whole cell lysate/extracC. 30 ug K562 whole cell lysate/extractD. 30 ug THP-1 whole cell lysate/extractE. 30 ug ...read more
Western Blot: Histone H3.3 Antibody [NBP2-14981] - A. 30 ug NIH-3T3 whole cell lysate/extractB. 30 ug JC whole cell lysate/extractC. 30 ug BCL-1 whole cell lysate/extractD. 30 ug C2C12 whole cell lysate/extractE. 30 ug ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pl, Po, Bv, Rb, Xp, ZeSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Histone H3.3 Antibody Summary

Immunogen
Full length human Histone H3.3 Recombinant protein.
Marker
Marker of Transcriptionally Active Chromatin
Predicted Species
Porcine (100%), Xenopus (100%), Rabbit (100%), Bovine (100%), Zebrafish (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H3F3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rice. Expected cross reactivity based on sequence homology: Xenopus laevis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H3.3 Antibody

  • H3.3A
  • H3F3
  • H3F3A
  • Histone H3.3
  • Histone H3.3A
  • Histone H3.3B
  • MGC87782
  • MGC87783

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Histone H3.3 NBP2-14981

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Bioinformatics

Gene Symbol H3F3A