Histone H3.1 Antibody

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-91814
    • Availability
      Product Discontinued

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Histone H3.1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to the N-terminal of Human Histone H3.
Specificity
Histone H3.1 (K4) detects endogenous levels of Histone H3.1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
H3C6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analysis of Histone H3.1 (K4) pAb in extracts from COLO205 cells.
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB100-91814.

Reactivity Notes

Human Mouse and Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H3.1 Antibody

  • H3 histone family, member D
  • H3.1HIST1H3D
  • H3/d
  • H3FA
  • H3FB
  • H3FC
  • H3FDHIST1H3F
  • H3FF
  • H3FH
  • H3FI
  • H3FJ
  • H3FK
  • H3FL
  • HIST1H3A
  • HIST1H3B
  • HIST1H3C
  • HIST1H3G
  • HIST1H3H
  • HIST1H3I
  • HIST1H3J
  • histone 1, H3e
  • histone cluster 1, H3e
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/i
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l

Background

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4 and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H1 is required for the condensation of nucleosome chains into higher order structures. Phosporylation of histone H1 is thought be involved in this process, although the exact nature of this role has yet to be elucidated. Evidence suggests that histone H1 is a part of a general repressor mechanism for stable repression of transcription, but it can also activate transcription of specific genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H3.1 Antibody (NB100-91814)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Research Areas for Histone H3.1 Antibody (NB100-91814)

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Blogs on Histone H3.1.

H3.1 - A core histone essential for genome storage and organization
Histones are the main protein component of chromatin and are essential for the storage and compaction of the genome. DNA wraps around histone oligomers to make up nucleosomes, the individual subunits of chromatin. By altering the accessibility of t...  Read full blog post.

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Bioinformatics

Gene Symbol H3C6
Entrez