Histone H2B [ac Lys15] Antibody - BSA Free

Western Blot: Histone H2B [ac Lys15] Antibody [NBP2-59272] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK15ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.

Immunocytochemistry/Immunofluorescence: Histone H2B [ac Lys15] Antibody [NBP2-59272] - HeLa cells were stained with the antibody against H2BK15ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2BK15ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Chromatin Immunoprecipitation: Histone H2B [ac Lys15] Antibody [NBP2-59272] - ChIP assays were performed using human HeLa cells, the antibody against H2BK15ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ELISA: Histone H2B [ac Lys15] Antibody [NBP2-59272] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2BK15ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:29,700.

• Reactivity: Hu
• Applications: WB, DB, ELISA, ICC/IF, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Histone H2B [ac Lys15] Antibody - BSA Free Summary

• Immunogen: The exact immunogen is proprietary information.
• Modification: ac Lys15
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H2BC18
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 2 ug
  • Chromatin Immunoprecipitation Sequencing
  • Dot Blot 1:20000
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300
• Purity: Peptide affinity purified

Alternate Names for Histone H2B [ac Lys15] Antibody - BSA Free

• FLJ35099
• FLJ56780
• FLJ56787
• HIST1H2BK
• histone 2, H2bf
• histone cluster 2, H2bf
• histone H2B type 2-F
• Histone H2B
• MGC131639

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility tothe cellular machineries which require DNA as a template. Histones thereby play a central role in transcriptionregulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set ofpost-translational modifications of histones, also called histone code, and nucleosome remodeling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2BC18