Histone H2B Antibody (1B3F12/A9)

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Western Blot: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]
Chromatin Immunoprecipitation: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]
Chromatin Immunoprecipitation: Histone H2B Antibody (1B3F12/A9) [NBP2-54770] - ChIP using anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]. Gcn5 regulates H2Bub and H3K9ac at several loci. ChIP-qPCR analysis of H2Bub ...read more
ELISA: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]

Product Details

Summary
Reactivity Hu, Y, YeSpecies Glossary
Applications WB, ChIP, ELISA, ICC/IF, ChIP
Clone
1B3F12/A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Histone H2B Antibody (1B3F12/A9) Summary

Description
This antibody was made against yeast monoubiquityl histone H2B and does not specifically reast with H2B or ubiquitin on other proteins. It detects endogenous levels of H2BK123ub1 in whole cell extracts and detects increased levels of H2BK123ub1 on native or tagged histoned H2B.
Immunogen
Ub-polypeptide antigen based on amino acids 115 - 130 of yeast H2B
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HIST2H2BF
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
Clone formerly called "H2BK123"
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-54770.

Reactivity Notes

Saccharomyces cerevisiae

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Notes

Clone formerly called "H2BK123"

Positive control(s): Yeast histone preparation.

Alternate Names for Histone H2B Antibody (1B3F12/A9)

  • FLJ35099
  • FLJ56780
  • FLJ56787
  • HIST1H2BK
  • histone 2, H2bf
  • histone cluster 2, H2bf
  • histone H2B type 2-F
  • Histone H2B
  • MGC131639

Background

C-terminus monoubiquitination of histone H2B acts as a signal for downstream processes such as gene regulation, genome packaging, histone modification crosstalk, and DNA repair. How the ubiquitination levels are controlled and how the Ub signal is translated to downstream effects is poorly understood. An antibody that detects K123 monoubiquitinated yeast H2B, homologue to human H2B K120, will enable research to discover the fundamental principles of the regulation and function of monoubiquitination of H2B. The antibody is developed using an ubiquitin-polypeptide antigen based on the amino-acids 115-130 of the yeast H2B protein, linked by a proteolytically stable bond.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H2B Antibody (NBP2-54770)(1)

We have publications tested in 1 confirmed species: Yeast.


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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HIST2H2BF
Entrez