Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) - BSA Free

Western Blot: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]

Chromatin Immunoprecipitation: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]

ELISA: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]

• Reactivity: Hu, Y, Ye
• Applications: WB, ELISA, ICC/IF, ChIP
• Clone: 1B3F12/A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) - BSA Free Summary

• Description: Clone formerly called "H2BK123"
• Immunogen: Ub-polypeptide antigen based on amino acids 115 - 130 of yeast Histone H2B
• Modification: ubiquitylated Lys123
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HIST2H2BF
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive control(s): Yeast histone preparation.
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Saccharomyces cerevisiae

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) - BSA Free

• FLJ35099
• FLJ56780
• FLJ56787
• HIST1H2BK
• histone 2, H2bf
• histone cluster 2, H2bf
• histone H2B type 2-F
• Histone H2B
• MGC131639

Background

C-terminus monoubiquitination of histone H2B acts as a signal for downstream processes such as gene regulation, genome packaging, histone modification crosstalk, and DNA repair. How the ubiquitination levels are controlled and how the Ub signal is translated to downstream effects is poorly understood. An antibody that detects K123 monoubiquitinated yeast H2B, homologue to human H2B K120, will enable research to discover the fundamental principles of the regulation and function of monoubiquitination of H2B. The antibody is developed using an ubiquitin-polypeptide antigen based on the amino-acids 115-130 of the yeast H2B protein, linked by a proteolytically stable bond.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone H2B Antibody (NBP2-54770)(1)

Publication using NBP2-54770

• Vlaming et al. Direct screening for chromatin status on DNA barcodes in yeast delineates the regulome of H3K79 methylation by Dot1. elife. 2016-12-07 [PMID: 27922451] (Yeast)

Bioinformatics

• Gene Symbol: HIST2H2BF