![Western Blot: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution. Positive control: Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates Lane 2: Untreated HeLa whole cel](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Western-Blot-NBP2-67336-img0009.jpg "Western Blot: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution. Positive control: Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates Lane 2: Untreated HeLa whole cel")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P |
| Clone | SR4-15 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein (SwissProt: P04908 Human
;SwissProt:Q93077 Human
;SwissProt: Q99878 Human
;SwissProt: P10812 Mouse
;SwissProt: P22752 Mouse
;SwissProt: P02262 Rat) |
| Modification | ac Lys9 |
| Localization | Nucleus, Chromosome. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HIST1H2AE |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.](http://images.novusbio.com/images/HIST1H4F-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBP2-07996-img0001.jpg)
![ELISA: H4/n Antibody (3E7H2) [NBP2-52486] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-ELISA-NBP2-52486-img0008.jpg)
![Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Western-Blot-NBP2-52486-img0009.jpg)
![Flow Cytometry: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Flow-Cytometry-NBP2-52486-img0001.jpg)
![Western Blot: HIST2H3C Overexpression Lysate (Adult Normal) [NBP2-06572] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST2H3C.](http://images.novusbio.com/images/HIST2H3C-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBP2-06572-img0001.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Analysis of Histone H2A in human PBMC lysate probed with Histone H2A antibody.](http://images.novusbio.com/images2/Histone-H2a-Antibody-Western-Blot-NB100-56346-img0002.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.](http://images.novusbio.com/images2/Histone-H2a-Antibody-Western-Blot-NB100-56346-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346] - Human breast probed with Histone H2A antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/images2/Histone-H2a-Antibody-Immunohistochemistry-Paraffin-NB100-56346-img0006.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/images2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0009.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/images2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0010.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining in human fallopian tube and skeletal muscle tissues using anti-CCDC6 antibody. Corresponding CCDC6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0012.jpg)
![Western Blot: HIST1H3C Overexpression Lysate (Adult Normal) [NBP2-07993] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H3C.](http://images.novusbio.com/images/HIST1H3C-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBP2-07993-img0001.jpg)
![Immunohistochemistry: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Staining of human prostate shows strong nuclear immunoreactivity in glandular and smooth muscle cells.](http://images.novusbio.com/images2/HIST1H2BC-Antibody-(CL5048)-Immunohistochemistry-NBP2-61153-img0001.jpg)
![Immunohistochemistry: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Staining of human fallopian tube shows strong nuclear immunoreactivity in glandular and stromal cells.](http://images.novusbio.com/images2/HIST1H2BC-Antibody-(CL5048)-Immunohistochemistry-NBP2-61153-img0002.jpg)
![Immunohistochemistry: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Staining of human duodenum shows strong nuclear positivity in glandular cells and in lamina propria cells.](http://images.novusbio.com/images2/HIST1H2BC-Antibody-(CL5048)-Immunohistochemistry-NBP2-61153-img0003.jpg)
![SDS-Page: Recombinant Human HIST1H4I Protein [H00008294-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00008294-P01-1.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2024-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NB21-2024] - 2 ug of NB21-2024 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Chromatin-Immunoprecipitation-NB21-2024-img0008.jpg)
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in HeLa histone prep.](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0009.jpg)
![Sandwich ELISA: H4/h Antibody (6D4) [H00008365-M01] - Detection limit for recombinant GST tagged HIST1H4H is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/images2/H4-h-Antibody-(6D4)-Sandwich-ELISA-H00008365-M01-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: H4/h Antibody (6D4) [H00008365-M01] - Analysis of monoclonal antibody to HIST1H4H on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/H4-h-Antibody-(6D4)-Immunocytochemistry-Immunofluorescence-H00008365-M01-img0002.jpg)
![Western Blot: H4/j Overexpression Lysate (Adult Normal) [NBL1-11567] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H4/j.](http://images.novusbio.com/images/H4-j-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBL1-11567-img0002.jpg)
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | HIST1H2AE |
![Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunocytochemistry-Immunofluorescence-NBP2-67336-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2AE antibody. The counter was dyed with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0012.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2AE antibody. The counter was dyed with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunocytochemistry-Immunofluorescence-NBP2-67336-img0001.jpg "Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunocytochemistry-Immunofluorescence-NBP2-67336-img0002.jpg "Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0004.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0005.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0006.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0007.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0008.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- Histone H2AE antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0010.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- Histone H2AE antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2AE antibody. The counter was dyed with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0011.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2AE antibody. The counter was dyed with hematoxylin.")
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Ovary, carcinoma](http://images.novusbio.com/images2/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0003.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Pancreas, carcinoma](http://images.novusbio.com/images2/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0004.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Colon, Carcinoma](http://images.novusbio.com/images2/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Histone-H3.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75524-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Histone-H3.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75524-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Histone-H3.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75524-img0003.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")