![Western Blot: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution. Positive control: Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates Lane 2: Untreated HeLa whole cel](http://images.novusbio.com/fullsize2/Histone H2AE [ac Lys9] Antibody (SR4-15)-Western Blot-NBP2-67336-img0009.jpg "Western Blot: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution. Positive control: Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates Lane 2: Untreated HeLa whole cel")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P |
| Clone | SR4-15 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide surrounding K9 of Histone H2A |
| Modification | ac Lys9 |
| Localization | Nucleus, Chromosome. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HIST1H2AE |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | HIST1H2AE |
![Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunocytochemistry-Immunofluorescence-NBP2-67336-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0008.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunocytochemistry-Immunofluorescence-NBP2-67336-img0001.jpg "Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunocytochemistry-Immunofluorescence-NBP2-67336-img0002.jpg "Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0004.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0005.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0006.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AE-[ac-Lys9]-Antibody-(SR4-15)-Immunohistochemistry-Paraffin-NBP2-67336-img0007.jpg "Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.")
![Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.](http://images.novusbio.com/images/HIST1H4F Overexpression Lysate (Adult Normal)-Western Blot-NBP2-07996-img0001.jpg)
![Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Western-Blot-NBP2-52486-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Immunohistochemistry-NBP2-52486-img0003.jpg)
![Immunohistochemistry-Paraffin: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of paraffin-embedded stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Immunohistochemistry-Paraffin-NBP2-52486-img0005.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/images2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Western-Blot-NBP1-30141-img0012.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Immunohistochemistry-Paraffin-NBP1-30141-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 [Trymethyl Lys9] (6F12-H4) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0011.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.](http://images.novusbio.com/images2/Histone-H2a-Antibody-Western-Blot-NB100-56346-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346] - Human breast probed with Histone H2A antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/images2/Histone-H2a-Antibody-Immunohistochemistry-Paraffin-NB100-56346-img0006.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Analysis of Histone H2A in human PBMC lysate probed with Histone H2A antibody.](http://images.novusbio.com/images2/Histone H2a Antibody-Western Blot-NB100-56346-img0002.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Lung, Non Small-Cell Carcinoma](http://images.novusbio.com/images2/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0006.jpg)
![Immunohistochemistry-Paraffin: Histamine H4 R Antibody [NLS3775] - IHC analysis of Lymph node using this antibody at 12 ug/ ml.](http://images.novusbio.com/images2/Histamine-H4-R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0001.jpg)
![Immunohistochemistry-Paraffin: Histamine H4 R Antibody [NLS3775] - Anti-HRH4 / Histamine H4 Receptor antibody IHC of human Ovary, Carcinoma and Inflammatory Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/images2/Histamine-H4-R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0002.jpg)
![Western Blot: CCDC6 Antibody [NBP1-85351] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/images2/CCDC6-Antibody-Western-Blot-NBP1-85351-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CCDC6 Antibody [NBP1-85351] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/CCDC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85351-img0013.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0021.jpg)
![Western Blot: Histone H3.1 Antibody [NBP2-75524] - Analysis of Histone H3.1 on F9 cell lysate using anti-Histone H3.1 antibody at 1/1,000 dilution.](http://images.novusbio.com/images2/Histone H3.1 Antibody-Western Blot-NBP2-75524-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Histone-H3.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75524-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H3.1 Antibody [NBP2-75524] - Analysis of paraffin-embedded human liver tissue using anti-Histone H3.1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Histone-H3.1-Antibody-Immunohistochemistry-Paraffin-NBP2-75524-img0004.jpg)
![Western Blot: HIST1H3C Overexpression Lysate (Adult Normal) [NBP2-07993] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H3C.](http://images.novusbio.com/images/HIST1H3C Overexpression Lysate (Adult Normal)-Western Blot-NBP2-07993-img0001.jpg)
![Western Blot: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Purified human recombinant histone H2B, H2A, H3 and H4.](http://images.novusbio.com/images2/HIST1H2BC-Antibody-(CL5048)-Western-Blot-NBP2-61153-img0007.jpg)
![Immunohistochemistry: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Staining of human kidney shows strong nuclear positivity in renal tubules and in glomerular cells.](http://images.novusbio.com/images2/HIST1H2BC-Antibody-(CL5048)-Immunohistochemistry-NBP2-61153-img0004.jpg)
![Western Blot: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Western blot analysis in human cell line HEL.](http://images.novusbio.com/images2/HIST1H2BC-Antibody-(CL5048)-Western-Blot-NBP2-61153-img0005.jpg)
![SDS-Page: Recombinant Human HIST1H4I Protein [H00008294-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00008294-P01-1.jpg)
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2024-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NB21-2024] - 2 ug of NB21-2024 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Chromatin-Immunoprecipitation-NB21-2024-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: H4/h Antibody (6D4) [H00008365-M01] - Analysis of monoclonal antibody to HIST1H4H on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/H4-h-Antibody-(6D4)-Immunocytochemistry-Immunofluorescence-H00008365-M01-img0002.jpg)
![Sandwich ELISA: H4/h Antibody (6D4) [H00008365-M01] - Detection limit for recombinant GST tagged HIST1H4H is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/images2/H4-h Antibody (6D4)-Sandwich ELISA-H00008365-M01-img0003.jpg)
![Western Blot: H4/j Overexpression Lysate (Adult Normal) [NBL1-11567] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H4/j.](http://images.novusbio.com/images/H4-j Overexpression Lysate (Adult Normal)-Western Blot-NBL1-11567-img0002.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")