Histone H2AE [ac Lys9] Antibody (SR4-15)

Western Blot: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution. Positive control: Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates Lane 2: Untreated HeLa whole cel

Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2AE antibody. The counter was dyed with hematoxylin.

Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Staining Histone H2A(acetyl K9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- Histone H2AE antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2AE [ac Lys9] Antibody (SR4-15) [NBP2-67336] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2AE antibody. The counter was dyed with hematoxylin.

Product Discontinued

Histone H2AE [ac Lys9] Antibody (SR4-15) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Histone H2AE aa aa 1-50 / 130 (acetyl K9) conjugated to Keyhole Limpet Haemocyanin (KLH). (SwissProt: P04908 Human; SwissProt:Q93077 Human; SwissProt: Q99878 Human; SwissProt: C0HKE6 Mouse; SwissProt: C0HKE1 Mouse; SwissProt: P02262 Rat)
• Modification: ac Lys9
• Localization: Nucleus, Chromosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HIST1H2AE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Histone H2AE [ac Lys9] Antibody (SR4-15)

• H2A histone family, member M
• H2A/m
• H2AFA
• H2AFMHistone H2A/m
• HIST1H2AE
• histone 1, H2ab
• histone cluster 1, H2ab
• histone H2A type 1-B/E
• Histone H2A.2
• Histone H2A/a

Background

Histone H2AE is 124 amino acids long, weighing approximately 13 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes and is a core component of the nucleosome. Current studies are being done on several diseases and disorders including riddle syndrome, systemic lupus erythematosus, lupus srythematosus, and immunodeficiency. This protein has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the proteasome degradation, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIST1H2AE