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![Western Blot: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Western blot analysis of various lysates using Histone H2AE Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-1012202411343397.jpg "Western Blot: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Western blot analysis of various lysates using Histone H2AE Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Western Blot: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Western blot analysis of various lysates using Histone H2AE Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-1012202411404011.jpg)
![Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Immunohistochemistry analysis of Histone H2AE in paraffin-embedded human small intestine tissue using Histone H2AE Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-31120251744480.jpg)
![Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Immunohistochemistry analysis of Histone H2AE in paraffin-embedded rat colon tissue using Histone H2AE Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-31120251744417.jpg)
![Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Immunohistochemistry analysis of Histone H2AE in paraffin-embedded rat brain tissue using Histone H2AE Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-31120251744454.jpg)
![Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Immunohistochemistry analysis of Histone H2AE in paraffin-embedded mouse colon tissue using Histone H2AE Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-3112025173158.jpg)
![Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Immunohistochemistry analysis of Histone H2AE in paraffin-embedded human esophagus tissue using Histone H2AE Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-31120251659369.jpg)
![Western Blot: Histone H2AE [p Ser1] Antibody (RM216) [NBP2-61489] - Western Blot of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole. Using NBP2-61489 at 0.5 ug/ml, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H2AE-p-Ser1-Antibody-RM216-Western-Blot-NBP2-61489-img0003.jpg "Western Blot: Histone H2AE [p Ser1] Antibody (RM216) [NBP2-61489] - Western Blot of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole. Using NBP2-61489 at 0.5 ug/ml, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.")
![Multiplex Immunoassay: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP2-61489] - RM216 specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).](http://images.novusbio.com/fullsize/nbp2-61489_rabbit-monoclonal-histone-h2ae-p-ser1-antibody-rm216-multiplex-immunoassay-18920252339436.png)
![Immunocytochemistry/ Immunofluorescence: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP2-61489] - Immunocytochemistry of HeLa cells, using Clone RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61489_rabbit-monoclonal-histone-h2ae-p-ser1-antibody-rm216-immunocytochemistry-immunofluorescence-18920252337120.png)
![Western Blot: Histone H2AE Antibody (RM225) [NBP2-61490] - Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using NBP2-61490 at 0.5 ug/ml.](http://images.novusbio.com/fullsize/Histone-H2AE-Antibody-RM225-Western-Blot-NBP2-61490-img0003.jpg "Western Blot: Histone H2AE Antibody (RM225) [NBP2-61490] - Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using NBP2-61490 at 0.5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Histone H2AE Antibody (RM225) [NBP2-61490] - Immunocytochemistry of HeLa cells, using NBP2-61490 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H2AE-Antibody-RM225-Immunocytochemistry-Immunofluorescence-NBP2-61490-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (3H5) [NBP3-15226] - Immunocytochemistry analysis of Histone H2AE antibody diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/Histone-H2AE-ac-Lys9-Antibody-3H5-Immunocytochemistry-Immunofluorescence-NBP3-15226-img0001.jpg "Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (3H5) [NBP3-15226] - Immunocytochemistry analysis of Histone H2AE antibody diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (3H5) [NBP3-15226] - Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30 minutes) with at Histone H2AE antibody 1:56, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Histone-H2AE-ac-Lys9-Antibody-3H5-Immunocytochemistry-Immunofluorescence-NBP3-15226-img0002.jpg)
![Western Blot: Histone H2AE Antibody [NBP1-79880] - RPMI-8226 Whole Cell, concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Histone-H2AE-Antibody-Western-Blot-NBP1-79880-img0002.jpg "Western Blot: Histone H2AE Antibody [NBP1-79880] - RPMI-8226 Whole Cell, concentration 1 ug/ml.")