Histone H2a Antibody

Product Discontinued

Histone H2a Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H2A. The immunogen region is: C-terminal.
• Specificity: Histone H2A (Ab-121) Antibody detects endogenous levels of total Histone H2A protein (UniProt IDs P0C0S8 / P04908 / P20671 / Q96KK5 / Q6FI13 / Q7L7L0).
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H2AC20
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100

Reactivity Notes

Human,Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Histone H2a Antibody

• H2A histone family, member Q
• H2A/q
• H2A1B
• H2AFQH2A
• H2A-GL101
• HIST1H2AB
• histone 2, H2ac
• histone cluster 2, H2ac
• histone H2A type 2-C
• Histone H2A
• Histone H2A/q
• Histone H2A-GL101
• histone IIa
• MGC74460

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Global mass spectrometric analysis of histone H2A variants/subtypes and their modifications have only recently been carried out. Nine histone H2A subtypes have been identified, including H2A2A and H2A2B. The two main H2A variants, H2AO and H2AC, as well as H2AL, were either acetylated at Lys 5 or phosphorylated at Ser 1. For the replacement histone H2AZ, acetylation at Lys 4 and Lys 7 was found.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2AC20