Histone H1.4 [p Thr17] Antibody (SR38-03)

Western Blot: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Analysis of Phospho-Histone H1.3(T17)+Histone H1.4(T17) on different cell lysates using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody at 1/500 dilution. Positive control: Lane 1: Untreated CRC whole cell lysates Lane2: CRC cells treated with

Immunocytochemistry/Immunofluorescence: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Histone H1.4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA

Immunocytochemistry/Immunofluorescence: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Analysis of paraffin-embedded mouse colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H1.4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocke

Immunohistochemistry-Paraffin: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Histone H1.4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked

Product Discontinued

Histone H1.4 [p Thr17] Antibody (SR38-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr17 of Human Histone H1.4. (SwissProt: P10412 Human; SwissProt: P16402 Human; SwissProt: P43274 Mouse; SwissProt: P43277 Mouse; Entrez Gene: 201097 Rat)
• Modification: p Thr17
• Localization: Nucleus, Chromosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HIST1H1E
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Histone H1.4 [p Thr17] Antibody (SR38-03)

• dJ221C16.5
• H1 histone family, member 4
• H1.4
• H1F4H1E
• histone 1, H1e
• histone cluster 1, H1e
• histone H1.4
• Histone H1b
• MGC116819

Background

The HIST1H1E gene encodes a 219 amino acid long, 21 kDA Histone H1.4 that functions by binding to linker DNA between nucleosome creating the macromolecular structure chromatin fiber. H1 histones as especially critical in condensation of nucleosome chains and the monitoring of individual gene transcription. HIST1H1E participates in PKA signaling, cell cycle start of DNA replication in early S phase, apoptosis, and histone modification. It interacts with genes HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4L. HIST1H1E has been researched regarding its role in progressive supranuclear palsy, pancreatitis, hepatitis, and x inactivation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIST1H1E