Histone H1 Antibody (AE-4)

Product Discontinued

Histone H1 Antibody (AE-4) Summary

• Immunogen: Nuclei from myeloid leukamia biopsy cells.
• Marker: Nuclear Marker
• Specificity: HISTONE H1 (AE-4)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: H1-1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100
• Application Notes: For IHC-Frozen sections: Acetone or PFA fixation is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: No buffer
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Histone H1 Antibody (AE-4)

• H1 histone family, member 1
• H1.1
• H1A
• H1F1HIST1
• histone 1, H1a
• histone cluster 1, H1a
• histone H1.1
• MGC126642
• MGC138345

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H1-1
• Entrez:
  • 3024(Human)
• Uniprot:
  • Q02539(Human)