GRPEL2 Antibody - BSA Free

Western Blot: GRPEL2 Antibody [NBP3-04966] - Analysis of extracts of various cell lines, using GRPEL2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP3-04966] - Immunohistochemistry of paraffin-embedded mouse kidney using GRPEL2 Rabbit pAb (NBP3-04966) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP3-04966] - Immunohistochemistry of paraffin-embedded rat kidney using GRPEL2 Rabbit pAb (NBP3-04966) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP3-04966] - Immunohistochemistry of paraffin-embedded human colon carcinoma using GRPEL2 Rabbit pAb (NBP3-04966) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GRPEL2 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-225 of human GRPEL2 (NP_689620.2). STATQRTAGEDCRSEDPPDELGPPLAERALRVKAVKLEKEVQDLTVRYQRAIADCENIRRRTQRCVEDAKIFGIQSFCKDLVEVADILEKTTECISEESEPEDQKLTLEKVFRGLLLLEAKLKSVFAKHGLEKLTPIGDKYDPHEHELICHVPAGVGVQPGTVALVRQDGYKLHGRTIRLARVEVAVESQRRL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRPEL2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GRPEL2 Antibody - BSA Free

• FLJ23713
• FLJ33918
• GrpE-like 2, mitochondrial (E. coli)
• Mt-GrpE#2mitochondrial

Background

Essential component of the PAM complex, a complex required for the translocation of transitpeptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems tocontrol the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Stimulates ATPase activity ofmt-HSP70. May also serve to modulate the interconversion of oligomeric (inactive) and monomeric (active) forms ofmt-HSP70

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRPEL2