GPR133 Overexpression Lysate (Native)

Images

 
Western Blot: GPR133 Overexpression Lysate (Adult Normal) [NBP2-04451] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPR133.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GPR133 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_942122. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens GPR133, mRNA.
Gene
GPR133

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
96.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GPR133

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GPR133 Overexpression Lysate (Native)

  • DKFZp434B1272
  • FLJ16770
  • G protein-coupled receptor 133
  • GPR133
  • G-protein coupled receptor PGR25
  • MGC138514
  • PGR25
  • PGR25MGC138512
  • probable G-protein coupled receptor 133

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NLS418
Species: Hu
Applications: IHC-P
AF4894
Species: Hu
Applications: WB, Neut
MAB8496
Species: Hu
Applications: Flow, CyTOF-ready
NLS1513
Species: Hu
Applications: IHC-P
H00347088-R02
Species: Hu
NBP1-89700
Species: Hu
Applications: IHC, IHC-P
PPS046
Species: Hu, Mu, Rt, Bv, Ca, Ch, GP, Pm, Ze
Applications: WB
H00442206-R02
Species: Hu
H00441931-R02
Species: Hu
NLS1720
Species: Hu
Applications: IHC-P
MAB194
Species: Hu, Mu
Applications: WB, Flow, CyTOF-ready, ICC
MAB8458
Species: Hu
Applications: Flow, CyTOF-ready, ICC
NBP1-02658
Species: Hu
Applications: ICC/IF (-), WB, IHC, IHC-P
NBP1-03561
Species: Hu
Applications: WB, IHC-P, PEP-ELISA
MAB4975
Species: Hu
Applications: IHC
NBP1-84903
Species: Hu
Applications: WB, IHC, IHC-P

Publications for GPR133 Lysate (NBP2-04451) (0)

There are no publications for GPR133 Lysate (NBP2-04451).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GPR133 Lysate (NBP2-04451) (0)

There are no reviews for GPR133 Lysate (NBP2-04451). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GPR133 Lysate (NBP2-04451) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GPR133 Products

Bioinformatics Tool for GPR133 Lysate (NBP2-04451)

Discover related pathways, diseases and genes to GPR133 Lysate (NBP2-04451). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GPR133 Lysate (NBP2-04451)

Discover more about diseases related to GPR133 Lysate (NBP2-04451).
 

Pathways for GPR133 Lysate (NBP2-04451)

View related products by pathway.

PTMs for GPR133 Lysate (NBP2-04451)

Learn more about PTMs related to GPR133 Lysate (NBP2-04451).
 

Research Areas for GPR133 Lysate (NBP2-04451)

Find related products by research area.

Blogs on GPR133

There are no specific blogs for GPR133, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GPR133 Antibody
NBP1-85142
GPR125 Antibody
NBP1-03561

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GPR133 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol GPR133
Entrez