GPR115 Antibody (527018) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: Flow
• Clone: 527018
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

GPR115 Antibody (527018) [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human GPR115
  Ser22-Ala347
  Accession # Q8IZF3
• Specificity: Detects human GPR115 in diect ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR115 Antibody (527018) [Alexa Fluor® 488]

• FLJ38076
• G protein-coupled receptor 115
• GPR115
• G-protein coupled receptor PGR18
• PGR18
• PGR18G-protein coupled receptor 115
• probable G-protein coupled receptor 115
• seven transmembrane helix receptor

Background

GPR115 is a member of the LN-7TM family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that show a long extracellular N-terminus (1, 2). The 695 amino acid (aa) human GPR115 sequence predicts a 21 aa signal sequence, a 385 aa N‑terminal extracellular domain (ECD), seven TM regions separated by 6‑24 aa intracellular and extracellular regions, and a 40 aa cytoplasmic tail. Like other LN-7TM members, the ECD contains a highly glycosylated mucin‑like stalk that is predicted to function in adhesion. This is followed by a cysteine-rich GPCR proteolytic cleavage site (GPS) (1). GPS domains, which have been described in other 7TM proteins including ETL, GPR126, HE6, and Latrophilin-1, are cleavage sites for processing proteins into two subunits (3‑7). Within the N‑terminal region that ends with the predicted cleavage site (aa 22‑347), human GPR115 shares 58% aa sequence identity with the corresponding region of mouse and rat GPR115. GPR115 was identified from expressed sequence tags (ESTs) found in pregnant uterus, breast, and the genitourinary tract (1).

1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
2. Bjarnadottir, T.K. et al. (2004) Genomics 84:23.
3. Nechiporuk, T. et al. (2001) J. Biol. Chem. 276:4150.
4. Moriguchi, T. et al. (2004) Genes Cells 9:549.
5. Kierszenbaum, A.L. (2003) Mol. Reprod. Dev. 64:1.
6. Krasnoperov, V.G. et al. (1997) Neuron 18:925.
7. Krasnoperov, V. et al. (2002) J. Biol. Chem. 277:46518.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8IZF3(Human)