GFPT2 Antibody

Immunocytochemistry/Immunofluorescence: GFPT2 Antibody [NBP2-38881] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.

Immunohistochemistry-Paraffin: GFPT2 Antibody [NBP2-38881] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.

Immunohistochemistry: GFPT2 Antibody [NBP2-38881] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: GFPT2 Antibody [NBP2-38881] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: GFPT2 Antibody [NBP2-38881] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: GFPT2 Antibody [NBP2-38881] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Western Blot: GFPT2 Antibody [NBP2-38881] -Analysis in human cell line U-251 MG.

Product Discontinued

GFPT2 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: PILYRTCTLENVKNICKTRMKRLDSSACL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GFPT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for GFPT2 Antibody

• D-fructose-6-phosphate amidotransferase 2
• FLJ10380
• GFAT 2
• GFAT2EC 2.6.1.16
• glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2
• glutamine: fructose-6-phosphate aminotransferase 2
• Glutamine:fructose 6 phosphate amidotransferase 2
• glutamine-fructose-6-phosphate transaminase 2
• Hexosephosphate aminotransferase 2

Background

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GFPT2
• Uniprot:
  • O94808(Human)