fd/M13 bacteriophage Antibody

Images

 
Lateral Flow Assay: fd/M13 bacteriophage Antibody [NB100-1633] - Norwalk VLPs in 100 uL are detected using anti-Norwalk antibodies in the test line (T); gold nanoparticle (top row) and antibody-phage construct followed ...read more
Detection of Norwalk VLPs in lateral-flow assay (LFA).Norwalk VLPs in 100 uL are detected using anti-Norwalk antibodies in the test line (T); gold nanoparticle (top row) and antibody-phage construct followed by ...read more

Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications ELISA, Flow, LFA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

fd/M13 bacteriophage Antibody Summary

Immunogen
Repeated injections with fd bacteriophage.
Specificity
The antibody binds specifically to phage coat proteins of fd phage or M13 phage.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VIII
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:1000 (indirect)
  • Flow Cytometry
  • Lateral Flow Assay
Application Notes
Use in FLOW reported in scientific literature (PMID: 27704069).
Publications
Read Publications using
NB100-1633 in the following applications:

Reactivity Notes

Cross-reacts with fd and M13 bacteriophages

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.01 M phosphate buffered saline, pH 7.4
Preservative
15mM Sodium Azide
Purity
IgG purified

Alternate Names for fd/M13 bacteriophage Antibody

  • major coat protein

Background

Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage.May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for fd/M13 bacteriophage Antibody (NB100-1633)(8)

We have publications tested in 2 confirmed species: Human, Virus.

We have publications tested in 2 applications: ELISA, FLOW.


Filter By Application
ELISA
(2)
FLOW
(5)
All Applications
Filter By Species
Human
(1)
Virus
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-1633 Applications Species
Wright, KM;DiNapoli, SR;Miller, MS;Aitana Azurmendi, P;Zhao, X;Yu, Z;Chakrabarti, M;Shi, W;Douglass, J;Hwang, MS;Hsiue, EH;Mog, BJ;Pearlman, AH;Paul, S;Konig, MF;Pardoll, DM;Bettegowda, C;Papadopoulos, N;Kinzler, KW;Vogelstein, B;Zhou, S;Gabelli, SB; Hydrophobic interactions dominate the recognition of a KRAS G12V neoantigen Nature communications 2023-08-21 [PMID: 37604828]
Hwang MS, Miller MS, Thirawatananond P et al. Structural engineering of chimeric antigen receptors targeting HLA-restricted neoantigens Nature communications 2021-09-06 [PMID: 34489470] (FLOW, ELISA) FLOW, ELISA
Kim J, Poling-Skutvik R, Trabuco Jr et al. Orientational binding modes of reporters in a viral-nanoparticle lateral flow assay. Analyst 2016-09-30 [PMID: 27704069] (FLOW) FLOW
Hagstrom AE, Garvey G, Paterson AS et al. Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay. PLoS One 2015-01-01 [PMID: 25978622] (Virus) Virus
Kim J BACTERIOPHAGE IMAGING IMMUNOASSAY FOR POINT OF CARE DIAGNOSTICS Dissertation 2016-08-01 (FLOW, Virus) FLOW Virus
Hwang MS, Mog BJ, Douglass J et al. Targeting loss of heterozygosity for cancer-specific immunotherapy Proceedings of the National Academy of Sciences of the United States of America 2021-03-23 [PMID: 33731480] (FLOW) FLOW
Hsiue EH, Wright KM, Douglass J et al. Targeting a neoantigen derived from a common TP53 mutation Science (New York, N.Y.) 2021-03-05 [PMID: 33649166]
Miller MS, Douglass J, Hwang MS et al. An engineered antibody fragment targeting mutant beta-catenin via Major Histocompatibility Complex I neoantigen presentation J. Biol. Chem. 2019-11-05 [PMID: 31690625] (FLOW, ELISA, Human) FLOW, ELISA Human

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol VIII
Uniprot