fd/M13 bacteriophage Antibody

Lateral Flow Assay: fd/M13 bacteriophage Antibody [NB100-1633] - Norwalk VLPs in 100 uL are detected using anti-Norwalk antibodies in the test line (T); gold nanoparticle (top row) and antibody-phage construct followed by HRP/anti-M13 conjugate (bottom row). Control line (C) consists of anti-mouse antibodies for the gold nanoparticle LFA and anti-M13 antibodies for the phage LFA. Nitrocellulose FF80HP was used as test membrane, Fusion 5 as sample pad and CF5 as absorbent pad. All images were equally gamma-corrected (gamma-correction factor = 0.45) to compensate for contrast lost in the overexposed, scanned images and better represent the naked-eye appearance of the raw strips. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0126571), licensed under a CC-BY licence.

• Reactivity: V-Vi
• Applications: ELISA, Flow, LFA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

fd/M13 bacteriophage Antibody Summary

• Immunogen: Repeated injections with fd bacteriophage.
• Specificity: The antibody binds specifically to phage coat proteins of fd phage or M13 phage.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VIII
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 (indirect)
  • Flow Cytometry
  • Lateral Flow Assay
• Application Notes: Use in FLOW reported in scientific literature (PMID: 27704069).

Reactivity Notes

Cross-reacts with fd and M13 bacteriophages

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.01 M phosphate buffered saline, pH 7.4
• Preservative: 15mM Sodium Azide
• Purity: IgG purified

Alternate Names for fd/M13 bacteriophage Antibody

• major coat protein

Background

Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage.May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for fd/M13 bacteriophage Antibody (NB100-1633)(7)

Publications using NB100-1633

• Hwang MS, Miller MS, Thirawatananond P et al. Structural engineering of chimeric antigen receptors targeting HLA-restricted neoantigens Nature communications 2021-09-06 [PMID: 34489470] (ELISA, FLOW)
• Kim J, Poling-Skutvik R, Trabuco Jr et al. Orientational binding modes of reporters in a viral-nanoparticle lateral flow assay. Analyst 2016-09-30 [PMID: 27704069] (FLOW)
• Hagstrom AE, Garvey G, Paterson AS et al. Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay. PLoS One 2015-01-01 [PMID: 25978622] (Virus)
• Kim J BACTERIOPHAGE IMAGING IMMUNOASSAY FOR POINT OF CARE DIAGNOSTICS Dissertation 2016-08-01 (FLOW, Virus)
• Hwang MS, Mog BJ, Douglass J et al. Targeting loss of heterozygosity for cancer-specific immunotherapy Proceedings of the National Academy of Sciences of the United States of America 2021-03-23 [PMID: 33731480] (FLOW)
• Hsiue EH, Wright KM, Douglass J et al. Targeting a neoantigen derived from a common TP53 mutation Science (New York, N.Y.) 2021-03-05 [PMID: 33649166]
• Miller MS, Douglass J, Hwang MS et al. An engineered antibody fragment targeting mutant beta-catenin via Major Histocompatibility Complex I neoantigen presentation J. Biol. Chem. 2019-11-05 [PMID: 31690625] (ELISA, FLOW, Human)

Bioinformatics

• Gene Symbol: VIII
• Uniprot:
  • P69541(Human)