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![Lateral Flow Assay: fd/M13 bacteriophage Antibody [NB100-1633] - Norwalk VLPs in 100 uL are detected using anti-Norwalk antibodies in the test line (T); gold nanoparticle (top row) and antibody-phage construct followed by HRP/anti-M13 conjugate (bottom row). Control line (C) consists of anti-mouse antibodies for the gold nanoparticle LFA and anti-M13 antibodies for the phage LFA. Nitrocellulose FF80HP was used as test membrane, Fusion 5 as sample pad and CF5 as absorbent pad. All images were equally gamma-corrected (gamma-correction factor = 0.45) to compensate for contrast lost in the overexposed, scanned images and better represent the naked-eye appearance of the raw strips. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0126571), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/fd-M13-bacteriophage-Antibody-Lateral-Flow-Assay-NB100-1633-img0001.jpg "Lateral Flow Assay: fd/M13 bacteriophage Antibody [NB100-1633] - Norwalk VLPs in 100 uL are detected using anti-Norwalk antibodies in the test line (T); gold nanoparticle (top row) and antibody-phage construct followed by HRP/anti-M13 conjugate (bottom row). Control line (C) consists of anti-mouse antibodies for the gold nanoparticle LFA and anti-M13 antibodies for the phage LFA. Nitrocellulose FF80HP was used as test membrane, Fusion 5 as sample pad and CF5 as absorbent pad. All images were equally gamma-corrected (gamma-correction factor = 0.45) to compensate for contrast lost in the overexposed, scanned images and better represent the naked-eye appearance of the raw strips. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0126571), licensed under a CC-BY license.")
![Western Blot: fd/M13 bacteriophage Antibody [NB100-1633] - Detection of Norwalk VLPs in lateral-flow assay (LFA).Norwalk VLPs in 100 μL are detected using anti-Norwalk antibodies in the test line (T); gold nanoparticle (top row) & antibody-phage construct followed by HRP/anti-M13 conjugate (bottom row). Control line (C) consists of anti-mouse antibodies for the gold nanoparticle LFA & anti-M13 antibodies for the phage LFA. Nitrocellulose FF80HP was used as test membrane, Fusion 5 as sample pad & CF5 as absorbent pad. All images were equally gamma-corrected (gamma-correction factor = 0.45) to compensate for contrast lost in the overexposed, scanned images & better represent the naked-eye appearance of the raw strips. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25978622), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1633_rabbit-polyclonal-fd-m13-bacteriophage-antibody-310202415392533.jpg)
![ELISA: fd/M13 bacteriophage Antibody (MM05) [NBP3-05788] - Coating: Coat corresponding Protein (20 ug/mL in pH 9.6 carbonate buffer) in NUNC plateSample: Add the indicated amounts of M13 Bacteriophages displaying Rabbit/Mouse Fab targeted for different proteins;Primary Antibody: M13 Antibody, 0.365 ug/mLSecondary Antibody: anti-mouse F(ab)2 Antibody/HRP](http://images.novusbio.com/fullsize/fd-M13-bacteriophage-Antibody-MM05-ELISA-NBP3-05788-img0001.jpg "ELISA: fd/M13 bacteriophage Antibody (MM05) [NBP3-05788] - Coating: Coat corresponding Protein (20 ug/mL in pH 9.6 carbonate buffer) in NUNC plateSample: Add the indicated amounts of M13 Bacteriophages displaying Rabbit/Mouse Fab targeted for different proteins;Primary Antibody: M13 Antibody, 0.365 ug/mLSecondary Antibody: anti-mouse F(ab)2 Antibody/HRP")
![ELISA: fd/M13 bacteriophage Antibody (PS00-01) [NBP3-32553] - Indirect ELISA analysis of fd/M13 bacteriophage was performed by coating HSA/BSA (2 μg/mL in pH 9.6 carbonate buffer) in 96-well plate, and then add the indicated amounts of fd/M13 Bacteriophages displaying anti-HSA Fab overnight at 4℃. Incubated with 100 µl per well of anti-M13 monoclonal antibody starting at a dilution of 1/2,500 and serially diluting it to a dilution of 1:160,000 for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32553_mouse-fd-m13-bacteriophage-mab-ps00-01-2782024114169.jpg "ELISA: fd/M13 bacteriophage Antibody (PS00-01) [NBP3-32553] - Indirect ELISA analysis of fd/M13 bacteriophage was performed by coating HSA/BSA (2 μg/mL in pH 9.6 carbonate buffer) in 96-well plate, and then add the indicated amounts of fd/M13 Bacteriophages displaying anti-HSA Fab overnight at 4℃. Incubated with 100 µl per well of anti-M13 monoclonal antibody starting at a dilution of 1/2,500 and serially diluting it to a dilution of 1:160,000 for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")