FARS2 Overexpression Lysate (Native)

Images

 
Western Blot: FARS2 Overexpression Lysate (Adult Normal) [NBL1-10595] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FARS2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FARS2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205843 and based on accession number NM_006567. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens phenylalanyl-tRNA synthetase 2, mitochondrial (FARS2), nuclear gene encoding mitochondrial protein, mRNA.
Gene
FARS2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
48.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for FARS2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FARS2 Overexpression Lysate (Native)

  • dJ236A3.1
  • dJ520B18.2 (FARS1 (phenylalanine-tRNA synthetase))
  • dJ520B18.2
  • EC 6.1.1
  • EC 6.1.1.20
  • FARS1dJ236A3.1 (phenylalanine-tRNA synthetase)
  • HSPC320
  • phenylalanine translase
  • phenylalanine tRNA ligase 2, mitochondrial
  • Phenylalanine--tRNA ligase
  • phenylalanine-tRNA synthetase 1 (mitochondrial)
  • phenylalanine-tRNA synthetase 2 (mitochondrial)
  • phenylalanyl-tRNA synthetase 2, mitochondrial
  • phenylalanyl-tRNA synthetase, mitochondrial
  • PheRS

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol FARS2