ERGI3 Antibody

Western Blot: ERGI3 Antibody [NBP2-76928] - Western blot analysis of ERGI3 on different lysates using anti-ERGI3 antibody at 1/500 dilution.Positive control:Lane1: SiHa Lane2: Human placentaLane3: Rat bone marrow

Immunocytochemistry/Immunofluorescence: ERGI3 Antibody [NBP2-76928] - ICC staining ERGI3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: ERGI3 Antibody [NBP2-76928] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

Flow Cytometry: ERGI3 Antibody [NBP2-76928] - Flow cytometric analysis of LOVO cells with ERGI3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: ERGI3 Antibody [NBP2-76928] - ICC staining ERGI3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: ERGI3 Antibody [NBP2-76928] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

Immunohistochemistry: ERGI3 Antibody [NBP2-76928] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

Immunohistochemistry: ERGI3 Antibody [NBP2-76928] - Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

Product Discontinued

ERGI3 Antibody Summary

• Immunogen: Synthetic peptide within Human ERGI3 aa 334-383 / 383. (SwissProt: Q9Y282 Human; SwissProt: Q9CQE7 Mouse)
• Localization: Endoplasmic reticulum. Golgi apparatus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERGIC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERGI3 Antibody

• C20orf47
• CGI-54
• chromosome 20 open reading frame 47
• dJ477O4.2
• endoplasmic reticulum-Golgi intermediate compartment protein 3
• endoplasmic reticulum-localized protein ERp43
• ERGIC and golgi 3
• ERV46
• NY-BR-84
• PRO0989
• SDBCAG84
• serologically defined breast cancer antigen 84
• Serologically defined breast cancer antigen NY-BR-84

Background

Possible role in transport between endoplasmic reticulum and Golgi (By similari

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERGIC3