Recombinant Human eEF1A1 Protein

SDS-Page: Recombinant Human eEF1A1 Protein [NBP2-51626] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human eEF1A1 Protein Summary

• Description: An un-tagged denatured recombinant protein corresponding to the amino acids 1-462 of Human eEF1A1
  
  

  Source: E.coli

  Amino Acid Sequence: MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK ITKSAQKAQK AK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EEF1A1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 50.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human eEF1A1 Protein

• CCS3
• CCS-3
• cervical cancer suppressor 3
• CTCL tumor antigen
• EE1A1
• EEF-1
• EEF1A
• eEF1A-1
• EF1AHNGC:16303
• EF1a-like protein
• EF-1-alpha-1
• EF-Tu
• elongation factor 1 alpha subunit
• elongation factor 1-alpha 1
• Elongation factor Tu
• Eukaryotic elongation factor 1 A-1
• eukaryotic translation elongation factor 1 alpha 1
• eukaryotic translation elongation factor 1 alpha 1-like 14
• FLJ25721
• glucocorticoid receptor AF-1 specific elongation factor
• GRAF-1EF
• LENG7
• leukocyte receptor cluster (LRC) member 7
• Leukocyte receptor cluster member 7
• MGC102687
• MGC131894
• MGC16224
• prostate tumor-inducing protein 1
• PTI1
• translation elongation factor 1 alpha 1-like 14

Background

The eEF1A1 gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for theenzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung,liver, kidney, and pancre

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: EEF1A1