eEF1A1 Antibody (JB44-13)

Western Blot: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution.Positive control:Lane 1: Rat brain tissueLane 2: Mouse skeletal muscleLane 3: Mouse cerebellumLane 4: Rat skin Lane 5: Daudi

Immunocytochemistry/Immunofluorescence: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Staining eEF1A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.

Flow Cytometry: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Analysis of THP-1 cells with eEF1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Staining eEF1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Staining eEF1A1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Analysis of paraffin-embedded human tonsil tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Analysis of paraffin-embedded human liver cancer tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Analysis of paraffin-embedded human kidney tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.

Product Discontinued

eEF1A1 Antibody (JB44-13) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human eEF1A1 aa 413-462 / 462. (SwissProt: P68104 Human; SwissProt: P10126 Mouse; SwissProt: P62630 Rat)
• Localization: Cell membrane, Cytoplasm, Membrane, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EEF1A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for eEF1A1 Antibody (JB44-13)

• CCS3
• CCS-3
• cervical cancer suppressor 3
• CTCL tumor antigen
• EE1A1
• EEF-1
• EEF1A
• eEF1A-1
• EF1AHNGC:16303
• EF1a-like protein
• EF-1-alpha-1
• EF-Tu
• elongation factor 1 alpha subunit
• elongation factor 1-alpha 1
• Elongation factor Tu
• Eukaryotic elongation factor 1 A-1
• eukaryotic translation elongation factor 1 alpha 1
• eukaryotic translation elongation factor 1 alpha 1-like 14
• FLJ25721
• glucocorticoid receptor AF-1 specific elongation factor
• GRAF-1EF
• LENG7
• leukocyte receptor cluster (LRC) member 7
• Leukocyte receptor cluster member 7
• MGC102687
• MGC131894
• MGC16224
• prostate tumor-inducing protein 1
• PTI1
• translation elongation factor 1 alpha 1-like 14

Background

The eEF1A1 gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for theenzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung,liver, kidney, and pancre

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EEF1A1