COLEC10 Antibody (4C1) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular and embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K and CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). Laminin and CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 um C. Laminin and CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006679), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular and embryonic localisation of CL-L1. D. CL-L1. Immunohistochemistry of a 18.5 days postfertilization mouse embryo. CL-L1 is expressed in the liver (long arrow) and submucosal patal region (short arrows). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006679), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular and embryonic localisation of CL-L1. Immunostaining of ATDC5 cells with the golgi marker 58K and CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow).Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006679), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular and embryonic localisation of CL-L1. Laminin and CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006679), licensed under a CC-BY license.

Immunohistochemistry: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular and embryonic localisation of CL-L1. Immunofluorescence showing co-localisation of CL-L1 and Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium and in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006679), licensed under a CC-BY license.

Sandwich ELISA: COLEC10 Antibody (4C1) [H00010584-M01] - Detection limit for recombinant GST tagged COLEC10 is 1 ng/ml as a capture antibody.

Immunohistochemistry-Paraffin: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular & embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K & CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin & CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 μm C. Laminin & CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) & submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 & Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium & in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28301481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular & embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K & CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin & CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 μm C. Laminin & CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) & submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 & Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium & in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28301481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular & embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K & CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin & CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 μm C. Laminin & CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) & submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 & Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium & in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28301481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular & embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K & CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin & CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 μm C. Laminin & CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) & submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 & Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium & in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28301481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular & embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K & CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin & CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 μm C. Laminin & CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) & submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 & Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium & in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28301481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: ELISA, ICC/IF, IHC
• Clone: 4C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

COLEC10 Antibody (4C1) - Azide and BSA Free Summary

• Immunogen: COLEC10 (NP_006429.1, 121 a.a. ~ 229 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CGRYRKFVGQLDISIARLKTSMKFVKNVIAGIRETEEKFYYIVQEEKNYRESLTHCRIRGGMLAMPKDEAANTLIADYVAKSGFFRVFIGVNDLEREGQYMFTDNTPLQ
• Specificity: COLEC10 - collectin sub-family member 10 (C-type lectin) (4C1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: COLEC10
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
• Application Notes: This product is useful for ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28301481).

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:28301481).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for COLEC10 Antibody (4C1) - Azide and BSA Free

• CL-34
• CL-L1
• CLL1collectin 34
• COLEC10
• collectin liver 1
• Collectin liver protein 1
• collectin sub-family member 10 (C-type lectin)
• Collectin-10
• Collectin-34
• MGC118794
• MGC118795

Background

This gene encodes a member of the C-lectin family, proteins that possess collagen-like sequences and carbohydrate recognition domains. The other members of this family are secreted proteins and bind to carbohydrate antigens on microorganisms facilitating their recognition and removal. This gene product is a cytosolic protein, a characteristic that suggests that it may have different biological functions than other C-lectins. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COLEC10 Antibody (H00010584-M01)(1)

Publication using H00010584-M01

• Munye MM, Diaz-Font A, Ocaka L et al. COLEC10 is mutated in 3MC patients and regulates early craniofacial development PLoS Genet 2017-03-01 [PMID: 28301481] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: COLEC10
• Entrez:
  • 10584(Human)
• Uniprot:
  • NP_006429()