Beta-Amylase Antibody [Biotin]

Dot Blot: Beta-Amylase Antibody [Biotin] [NB120-20638] - Dot Blot results of Rabbit Beta-Amylase antibody [Biotin].Antigen: Beta-Amylase.Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng.Blocking Buffer for 30 minutes at RT.Primary Antibody: Rabbit Anti-Beta-Amylase Biotin 1ug/mL for 1hr at RT.Secondary Antibody: Goat anti-Rabbit HRP at 1:70,000 for 30min at RT.Imaging System ChemiDoc, Filter used: Chemi.

• Reactivity: Pl
• Applications: WB, DB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Biotin
• Concentration: LYOPH

Beta-Amylase Antibody [Biotin] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b-Amylase [Sweet Potato]
• Immunogen: Beta-Amylase [Sweet Potato] (Uniprot: P10537)
• Localization: Cytoplasmic
• Specificity: Cross reactivity against b Amylase from other sources may occur but has not been specifically determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:5000-1:25000
  • Western Blot 1:500-1:3000
• Application Notes: This product has been tested by dot blot and western blot. This product is assayed against 1.0 ug of b-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Cross reactivity against Beta Amylase from other sources may occur but has not been specifically determined.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Beta-Amylase Antibody [Biotin]

• 14 alpha D glucan maltohydrolase
• 1-4 alpha D glucan maltohydrolase
• AMYB
• BMY1

Background

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P10537(Plant)