Ascorbate Oxidase Antibody [Biotin]

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Product Details

Summary
Reactivity PlSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin
Concentration
LYOPH

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Ascorbate Oxidase Antibody [Biotin] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Ascorbate Oxidase [cucurbita]. Uniprot ID P24792
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Delipidation and Defibrination
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
Readout System

Reactivity Notes

Reactive with Ascorbate Oxidase from Cucurbita spp.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Delipidation and Defibrination
Reconstitution Instructions
Reconstitute with 100 ul deionized water

Alternate Names for Ascorbate Oxidase Antibody [Biotin]

  • Ascorbase
  • ASO
  • L ascorbate oxidase precursor

Background

Ascorbate Oxidase is a copper-containing enzyme. It catalyzes the following reaction: Ascorbic acid + O2 -----> Dehydroascorbic acid + H2O. Ascorbate Oxidase is widely distributed and is only present in plants. Its biological role in plants is still not clear. Studies after increasing and reducing the enzyme activity suggest that it has a positive effect on plant growth. In addition, it has been proposed that the enzyme may be involved in plant defense by modifying the ascorbate level and thus redox state in apoplast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Ascorbate Oxidase Products

Array NB100-2004

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