AP1M1 Knockout HeLa Cell Lysate

Product Discontinued

AP1M1 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,25 bp insertion in exon1 and 26 bp insertion in exon1 and Insertion of the selection cassette in exon1
• Gene: AP1M1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for AP1M1 Knockout HeLa Cell Lysate

• Adaptor protein complex AP-1 mu-1 subunit
• Adaptor-related protein complex 1 mu-1 subunit
• adaptor-related protein complex 1, mu 1 subunit
• AP47MU-1A
• AP-mu chain family member mu1A
• CLAPM2AP-1 complex subunit mu-1
• clathrin adaptor protein AP47
• Clathrin assembly protein complex 1 medium chain 1
• clathrin assembly protein complex 1, medium chain
• clathrin assembly protein complex AP1, mu subunit
• Clathrin coat assembly protein AP47
• Clathrin coat-associated protein AP47
• CLTNM
• golgi adaptor AP-1 47 kDa protein
• Golgi adaptor HA1/AP1 adaptin mu-1 subunit
• HA1 47 kDa subunit
• mu1A-adaptin
• Mu-adaptin 1

Background

The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: AP1M1