AP1G2 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

AP1G2 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human AP1G2.
  
  

  Source: E. coli

  Amino Acid Sequence: PLVERDGPQADEEAKESKEAAQLSEAAPVPTEPQASQLLDLLDLLDGASGDVQHPPHLDPSPGGALVHLLDLPCVPPPPAPIPDLKVFEREGVQLNLSFIRPPENPALLLITITATNFSEGDV

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: AP1G2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-81857.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for AP1G2 Recombinant Protein Antigen

• adaptor-related protein complex 1, gamma 2 subunit
• AP-1 complex subunit gamma-like 2
• clathrin-associated/assembly/adaptor protein, large, gamma-2
• G2AD
• gamma2-adaptin

Background

AP1G2, also known as AP-1 complex subunit gamma-like 2, consists of a 785 amino acid isoform that is 87 kDa, and is involved in protein sorting and transferring molecules between the trans-Golgi network and the surface of the cell. Disease research is currently being conducted on the relation between AP1G2 and malaria, sarcoma, hepatitis, immunodeficiency, and Kaposi's sarcoma. The protein interacts with RABEP1, XRN1, AP1S1, SYNRG, and NEDD4 in the Transport Clathrin-coated vesicle cycle and Clathrin-dependent protein traffic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: AP1G2