![Western Blot: AKD1 Antibody [NBP2-33936] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/AKD1-Antibody-Western-Blot-NBP2-33936-img0004.jpg "Western Blot: AKD1 Antibody [NBP2-33936] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit AKD1 Antibody - BSA Free (NBP2-33936) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: VMAEHNPQYLIELNGNKPAEELFMIVMDRLKYLNLKRAAILTKLQGAEEEINDTMENDELFRTLASYKLIAPRYRWQRSKWG |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AK9 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
||
| Control Peptide |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for AKD1 Antibody (NBP2-33936)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: AKD1 Antibody [NBP2-33936] - Staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/AKD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33936-img0005.jpg "Immunocytochemistry/Immunofluorescence: AKD1 Antibody [NBP2-33936] - Staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: AKD1 Antibody [NBP2-33936] - Staining in human testis and liver tissues using anti-AK9 antibody. Corresponding AK9 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AKD1-Antibody-Immunohistochemistry-Paraffin-NBP2-33936-img0008.jpg "Immunohistochemistry-Paraffin: AKD1 Antibody [NBP2-33936] - Staining in human testis and liver tissues using anti-AK9 antibody. Corresponding AK9 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: AKD1 Antibody [NBP2-33936] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/AKD1-Antibody-Immunohistochemistry-Paraffin-NBP2-33936-img0006.jpg "Immunohistochemistry-Paraffin: AKD1 Antibody [NBP2-33936] - Staining of human liver shows low expression as expected.")
![Immunohistochemistry-Paraffin: AKD1 Antibody [NBP2-33936] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/AKD1-Antibody-Immunohistochemistry-Paraffin-NBP2-33936-img0007.jpg "Immunohistochemistry-Paraffin: AKD1 Antibody [NBP2-33936] - Staining of human testis shows high expression.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")