Aflatoxin B1 Antibody (6A10) [CoraFluor™ 1]

• Reactivity: Hu, All
• Applications: WB, DB, ELISA, ICC/IF, IHC
• Clone: 6A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Aflatoxin B1 Antibody (6A10) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Aflatoxin B1 DNA (against the midazole ring-opened persistent form of the major N-7 guanine adduct of AFB1).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Aflatoxin B1 Antibody (6A10) [CoraFluor™ 1]

• AFB1
• AFB1-AR1
• Aldoketoreductase 7

Background

Aflatoxin B1 is a naturally occurring mycotoxin produced by two types of mold: Aspergillus flavus and Aspergillus parasiticus. These molds commonly affect grains which are grown under stressful conditions such as drought and also grows on food that is stored in hot and humid conditions.

There are 13 known Aflatoxins produced, with aflatoxin B1 considered as the most toxic. In fact, Aflatoxin B1 is the most potent hepatocellular carcinoma (HCC) causing chemical currently known. Specifically, Aflatoxin B1 is thought to case mutations in the p53 gene which interfere with the gene's critical tumor suppressing functions.

Aflatoxin B1 are useful tools for studying hepatocellular carcinoma formation with wide ranging implications into public health and food saftey.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.