Aflatoxin B1 Antibody (6A10)


Immunohistochemistry: Aflatoxin B1 Antibody (6A10) [NB600-443] - Immuno staining of positive (A) and negative (B) tumor tissues for AFB, -DNA adducts. Photos courtesy of Dr. Regina Santella.

Product Details

Reactivity AllSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
1.5 mg/ml

Aflatoxin B1 Antibody (6A10) Summary

Aflatoxin B1 DNA (against the midazole ring-opened persistent form of the major N-7 guanine adduct of AFB1).
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1% Sodium Azide
1.5 mg/ml
Protein G purified


Application Notes
This Aflatoxin B1 antibody is useful for ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence and Western blot. Immunohistochemistry-Frozen, Dot Blot and Immunohistochemistry-Paraffin were reported in scientific literature.
Reviewed Applications
Read 2 Reviews rated 4.5
NB600-443 in the following application:

Read Publications using
NB600-443 in the following applications:

Reactivity Notes

All species. There is no cross-reactivity with other carcinogen-DNA adducts.

Alternate Names for Aflatoxin B1 Antibody (6A10)

  • AFB1
  • AFB1-AR1
  • Aldoketoreductase 7


Aflatoxins (AF) are a group of harmful fungal toxins commonly found as grains/groundnuts contaminants and the most abundant congeners of AF, aflatoxin B1 (AFB1) is the most toxic among AFs which is produced by Aspergillus flavus, A. parasiticus etc. Humans and domestic animals can be exposed to aflatoxins by foods and approximately 25% of the world's food supply is annually contaminated with mycotoxins indicating the widespread prevalence of this public health problem. AFB1, a potent hepatotoxin/hepatocarcinogen, undergoes metabolism catalyzed by mixed function oxygenases of hepatic cytochrome P-450 enzyme system to produce a highly reactive intermediate, aflatoxin B1-8,9 epoxide that binds to necleophilic sites in hepatocyte DNA to form a number of adducts. The reactive aflatoxin B1-8,9 epoxide attracts guanine residues in double stranded DNA and the adducts formation leads to initiation of hepatocarcinogenesis. ROS generated during aflatoxins metablosm are considered to be main culprit for hepatotoxicity. The AFB1-8,9-epoxide can further be converted to AFB1-8,9-diol that specifically binds to lysine in albumin and forms AFB1-lysine adducts (AFB-Lys), which has been validated as a biomarker of human aflatoxins exposure.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aflatoxin B1 Antibody (NB600-443)(18)

We have publications tested in 2 confirmed species: Human, Zebrafish.

We have publications tested in 7 applications: DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB600-443 Applications Species
Qureshi H, Hamid SS, Ali SS et al. Cytotoxic effects of aflatoxin B1 on human brain microvascular endothelial cells of the blood-brain barrier Med. Mycol. 2015 May 01 [PMID:25851265] (DB) DB
Liu Yi-Xiao, Long Xi-Dai, Xi Zhi-Feng et al. MicroRNA-24 modulates aflatoxin B1-related hepatocellular carcinoma prognosis and tumorigenesis. Biomed Res Int. 2014 Apr 08 [PMID:24800232] (ELISA, Human) ELISA Human
Qi LN, Bai T, Chen ZS et al. The p53 mutation spectrum in hepatocellular carcinoma from Guangxi, China : role of chronic hepatitis B virus infection and aflatoxin B1 exposure. Liver Int. 2014 Jan 27 [PMID:24461059] (IHC-P) IHC-P
Qi LN, Li LQ, Chen YY et al. Genome-Wide and Differential Proteomic Analysis of Hepatitis B Virus and Aflatoxin B1 Related Hepatocellular Carcinoma in Guangxi, China. PLoS One 2013 Dec 31 [PMID:24391771] (IHC-P) IHC-P
Huang XY, Yao JG, Huang HD et al. MicroRNA-429 Modulates Hepatocellular Carcinoma Prognosis and Tumorigenesis. Gastroenterol Res Pract. 2013 [PMID:24204382] (ELISA) ELISA
Zhang Z, Lu H, Huan F et al. Cytochrome P450 2A13 mediates the neoplastic transformation of human bronchial epithelial cells at a low concentration of aflatoxin B1. Int J Cancer 2013 Sep 30 [PMID:24114584] (ICC/IF) ICC/IF
Yang X, Zhang Z, Wang X et al. Cytochrome P450 2A13 enhances the sensitivity of Human bronchial epithelial cells to aflatoxin B1-induced DNA damage. Toxicol Appl Pharmacol 2013 Apr 18 [PMID:23602888] (ICC/IF) ICC/IF
Lu JW, Yang WY, Lin YM et al. Hepatitis B virus X antigen and aflatoxin B1 synergistically cause hepatitis, steatosis and liver hyperplasia in transgenic zebrafish Acta Histochem 2013 Mar 14 [PMID:23499292] (IHC-P, Zebrafish) IHC-P Zebrafish
Yang XJ, Lu HY, Li ZY et al. Cytochrome P450 2A13 mediates aflatoxin B1-induced cytotoxicity and apoptosis in human bronchial epithelial cells Toxicology 2012 Jun 26 [PMID:22743290] (IHC, Human) IHC Human
Shirabe K, Toshima T, Taketomi A et al. Hepatic aflatoxin B1-DNA adducts and TP53 mutations in patients with hepatocellular carcinoma despite low exposure to aflatoxin B1 in southern Japan. Liver Int. 2011 Jun 28. [PMID:21745313] (IHC-P) IHC-P
Show All 18 Publications.

Reviews for Aflatoxin B1 Antibody (NB600-443) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.
We have 2 reviews tested in 2 application: IHC, ELISA.

Reviews using NB600-443:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
reviewed by:
IHC Other 12/09/2011


Sample TestedZebra Fish


Blocking DetailsBlocking Buffer: 5% FBS, Blocking Time: 1 hour

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-mouse HRP


Detection NotesDetection Visualization Method: Invitrogen
reviewed by:
ELISA Mouse 09/08/2010


Sample Testedmouse lung tissue

Product General Protocols

View specific protocols for Aflatoxin B1 Antibody (NB600-443): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aflatoxin B1 Antibody (NB600-443). (Showing 1 - 1 of 1 FAQs).

  1. I have an inquiry about NB600-443. The product name on your homepage states, that this antibody is against Aflatoxin B1, but the Immunogen states to be Aflatoxin B1 DNA - could you please clarify if this antibody recognizes the aflatoxin B1 toxin or the DNA of Aflatoxin B1? In case this antibody recognizes the toxin itself - are you aware of another antibody in your portfolio that has successfully been used with NB600-443 in a Sandwich ELISA?
    • I can inform you that Aflatoxin B1 Antibody (6A10) (NB600-443) is specific for aflatoxin B1 DNA adducts (DNA modified with the toxin; particularly the imidazole ring-opened form), and is not suitable for detecting free aflatoxin B1. Please see this paper for a detailed description of the clone 6A10: Cancer Research.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB600-443AF405
Alexa Fluor (R) 488 Labeled NB600-443AF488
Alexa Fluor (R) 647 Labeled NB600-443AF647
Alexa Fluor (R) 700 Labeled NB600-443AF700
Allophycocyanin Labeled NB600-443APC
Biotin Labeled NB600-443B
DyLight 350 Labeled NB600-443UV
DyLight 405 Labeled NB600-443V
DyLight 405LS Labeled NB600-443V3
DyLight 488 Labeled NB600-443G
DyLight 550 Labeled NB600-443R
DyLight 650 Labeled NB600-443C
DyLight 680 Labeled NB600-443FR
DyLight 755 Labeled NB600-443IR
FITC Labeled NB600-443F
HRP Labeled NB600-443H
PE Labeled NB600-443PE
PerCP Labeled NB600-443PCP

Additional Aflatoxin B1 Antibody (6A10) Products

Research Areas for Aflatoxin B1 Antibody (NB600-443)

Find related products by research area.

Blogs on Aflatoxin B1.

Using Aflatoxin B1 Antibody for Hepatocellular Carcinoma Studies
We at Novus Biologicals are constantly updating our antibody catalog in order to provide as comprehensive a database as possible for molecular biology researchers. Not all our antibodies are derived from proteins found in mammalian or human tissue. So...  Read full blog post.

Contact Information

Product PDFs

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB600-443 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought