ACBD4 Overexpression Lysate (Native)


Western Blot: ACBD4 Overexpression Lysate (Adult Normal) [NBL1-07227] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ACBD4.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

ACBD4 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC206781 and based on accession number NM_024722. The protein contains a C-terminal DDK tag.
C-terminal Myc/DDK
Homo sapiens acyl-Coenzyme A binding domain containing 4 (ACBD4), transcript variant 2, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
34.6 kDa

Lysate Details for ACBD4

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ACBD4 Overexpression Lysate (Native)

  • acyl-CoA binding domain containing 4
  • acyl-CoA-binding domain-containing protein 4
  • acyl-Coenzyme A binding domain containing 4
  • FLJ13322
  • FLJ90623


This gene encodes a member of the acyl-coenzyme A binding domain containing protein family. All family members contain the conserved acyl-Coenzyme A binding domain, which binds acyl-CoA thiol esters. They are thought to play roles in acyl-CoA dependent lipid metabolism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2) with a distinct C-terminus, compared to isoform 1. Variants 2 and 4 encode the same protein (isoform 2).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Contact Information

Product PDFs


Gene Symbol ACBD4

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