VEGF-D Antibodies

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " alt="VEGF‑D was detected in immersion fixed paraffin-embedded sections of Human Pancreas using Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB286) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " class="big_thumb" />

622-VD) was serially diluted and captured by Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB2861) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB286) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 622-VD) was serially diluted and captured by Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB2861) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB286) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in pneumocytes in lung. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in pneumocytes in lung. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="VEGF-D was detected in immersion fixed paraffin-embedded sections of mouse lung tissue using Goat Anti-Mouse VEGF-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF469) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in pneumocytes in lung. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

469-VD) was serially diluted and captured by Rat Anti-Mouse VEGF‑D Monoclonal Antibody (Catalog # MAB469) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse VEGF‑D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF469) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 469-VD) was serially diluted and captured by Rat Anti-Mouse VEGF‑D Monoclonal Antibody (Catalog # MAB469) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse VEGF‑D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF469) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

622-VD) stimulates proliferation in HMVEC human microvascular endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF-D (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF286). The ND₅₀ is typically 3-10 µg/mL." class="big_lightbox"> 622-VD) stimulates proliferation in HMVEC human microvascular endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF-D (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF286). The ND₅₀ is typically 3-10 µg/mL." alt="Recombinant Human VEGF-D (622-VD) stimulates proliferation in HMVEC human microvascular endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF-D (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF286). The ND₅₀ is typically 3-10 µg/mL." class="big_thumb" />

622-VD) was serially diluted and captured by Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB2861) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB286) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 622-VD) was serially diluted and captured by Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB2861) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB286) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human VEGF‑D (Catalog # 622-VD) was serially diluted and captured by Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB2861) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB286) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

469-VD) was serially diluted and captured by Rat Anti-Mouse VEGF‑D Monoclonal Antibody (Catalog # MAB469) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse VEGF‑D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF469) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 469-VD) was serially diluted and captured by Rat Anti-Mouse VEGF‑D Monoclonal Antibody (Catalog # MAB469) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse VEGF‑D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF469) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse VEGF‑D (Catalog # 469-VD) was serially diluted and captured by Rat Anti-Mouse VEGF‑D Monoclonal Antibody (Catalog # MAB469) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse VEGF‑D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF469) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />