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![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can response to both Pam3CSK4 (TLR2/TLR1 specific ligand) and MALP-2 (TLR2/TLR6 specific ligand). The TLR2 / TLR4 inhibitor specifically inhibits TLR2/TLR1 receptor complex activity in a dose-response manner but exhibited no or little effect on TLR2/TLR6 receptor complex activity.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0007.jpg "TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can response to both Pam3CSK4 (TLR2/TLR1 specific ligand) and MALP-2 (TLR2/TLR6 specific ligand). The TLR2 / TLR4 inhibitor specifically inhibits TLR2/TLR1 receptor complex activity in a dose-response manner but exhibited no or little effect on TLR2/TLR6 receptor complex activity.")
![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - The TLR4 reporter cell line responds to LPS. The TLR2 / TLR4 inhibitor specifically inhibits TLR4 activation upon LPS stimulation in a dose-response manner.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0008.jpg)
![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 /4 Inhibitor suppressed the Pam3CSK4-induced TLR2/TLR1 activity [A] and the LPS-induced TLR4 activity [B] in a dose-response manner, of which IC50 values were measured as 17.2 uM and 10.47 uM, respectively.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0009.jpg)
![Functional (Inhibition): TIRAP (TLR2 and TLR4) Inhibitor Peptide Set [NBP2-29331] - TLR2/NF-kB/SEAPorter HEK 293 (NBP2-26274) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29331) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Pam3CSK4 (NBP2-25297) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-29331-img0001.jpg "Functional (Inhibition): TIRAP (TLR2 and TLR4) Inhibitor Peptide Set [NBP2-29331] - TLR2/NF-kB/SEAPorter HEK 293 (NBP2-26274) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29331) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Pam3CSK4 (NBP2-25297) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).")
![Western Blot: TIRAP (TLR2 and TLR4) Overexpression Lysate (Native) [NBP2-08583] - TIRAP (TLR2 and TLR4) Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIRAP.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Overexpression-Lysate-Native-Western-Blot-NBP2-08583-img0002.jpg "Western Blot: TIRAP (TLR2 and TLR4) Overexpression Lysate (Native) [NBP2-08583] - TIRAP (TLR2 and TLR4) Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIRAP.")
![SDS-Page: Recombinant Human TIRAP (TLR2 and TLR4) Protein [NBP2-22871] -](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Protein-SDS-Page-NBP2-22871-img0002.jpg "SDS-Page: Recombinant Human TIRAP (TLR2 and TLR4) Protein [NBP2-22871] -")
![Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Antibody-Western-Blot-NB300-990-img0005.jpg "Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: TIRAP Antibody [NB100-2823] - Staining (0.3µg/ml) of A549 (A) and K562 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TIRAP-Antibody-Western-Blot-NB100-2823-img0004.jpg "Western Blot: TIRAP Antibody [NB100-2823] - Staining (0.3µg/ml) of A549 (A) and K562 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: TIRAP Antibody [NB100-2823] - Staining (0.3ug/ml) of Human colorectal cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TIRAP-Antibody-Western-Blot-NB100-2823-img0005.jpg)