ST2/IL-33R Antibodies

HAF008). A specific band was detected for ST2/IL-33 R at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for ST2/IL-33 R at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human kidney tissue and small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5233) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ST2/IL-33 R at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) human ST2/IL-33 R or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5232) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

DY990). Mouse Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5231) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Mouse Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5231) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

MAB5232) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5231) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB5232) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5231) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human ST2/IL-33 R protein was serially diluted 2-fold and captured by Rabbit Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5232) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5231) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />