PKM2 Antibodies

HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A549 human lung carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat PKM1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Sertoli cells in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Sertoli cells in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Sertoli cells in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Sertoli cells in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="PKM2 was detected in immersion fixed WI-38 human lung fibroblast cell line using Mouse Anti-Human/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB7244) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to epithelial cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to epithelial cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control.. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control.. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A549 human lung carcinoma cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB72442) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="PKM2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human PKM1/2 Monoclonal Antibody (Catalog # MAB72443) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />