IL-4 Antibodies

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplams in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplams in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL‑4 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Mouse Anti-Human IL‑4 Monoclonal Antibody (Catalog # MAB2043) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplams in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

6939-RB) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Proliferation elicited by IL-4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rabbit IL-4 Monoclonal Antibody (Catalog # MAB6939). The ND₅₀ is typically 0.25-1.25 µg/mL." class="big_lightbox"> 6939-RB) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Proliferation elicited by IL-4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rabbit IL-4 Monoclonal Antibody (Catalog # MAB6939). The ND₅₀ is typically 0.25-1.25 µg/mL." alt="Recombinant Rabbit IL-4 (Catalog # 6939-RB) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Proliferation elicited by IL-4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rabbit IL-4 Monoclonal Antibody (Catalog # MAB6939). The ND₅₀ is typically 0.25-1.25 µg/mL." class="big_thumb" />

654-P4) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-4 (30 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Porcine IL-4 Monoclonal Antibody (Catalog # MAB6542). The ND₅₀ is typically 0.3-1.2 µg/mL." class="big_lightbox"> 654-P4) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-4 (30 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Porcine IL-4 Monoclonal Antibody (Catalog # MAB6542). The ND₅₀ is typically 0.3-1.2 µg/mL." alt="Recombinant Porcine IL-4 (Catalog # 654-P4) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-4 (30 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Porcine IL-4 Monoclonal Antibody (Catalog # MAB6542). The ND₅₀ is typically 0.3-1.2 µg/mL." class="big_thumb" />

204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB204R). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_lightbox"> 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB204R). The ND₅₀ is typically 0.5-1.5 µg/mL." alt="Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB204R). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_thumb" />

F0101B) and then Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> F0101B) and then Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="PBMC lymphocytes treated with Tocris Cell Activation Cocktail and Brefeldin A (A) vs PBMC lymphocytes treated with Brefeldin A (B) were stained with Mouse Anti-Human IL‑4 Monoclonal Antibody (Catalog # mab11474) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and then Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />