| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43312-0.5mg | |||
| BSA Free | NBP1-43312-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08856 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05194-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21662-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25146SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Atto594 | NBP3-14765-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1338-SP | |||
![Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the PE conjugate of NBP1-43312. Analysis of mouse splenocyte suspensions using MHC Class II PE and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0009.jpg "Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the PE conjugate of NBP1-43312. Analysis of mouse splenocyte suspensions using MHC Class II PE and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.")
![Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Staining of C57BL/6 splenocytes with 0.06 ug of Rat IgG2b K Isotype Control Purified (cat. NBP1-43323) (open histogram) or 0.06 ug of Anti-Mouse MHC Class II (I-A/I-E) Purified (filled histogram) followed by Anti-Rat IgG FITC (cat. NBP1-69751). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0001.jpg)
![Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the Allophycocyanin conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC and 0.015 micrograms of Rat IgG2b ? Isotype Control APC (left) or 0.015 micrograms of Anti-Mouse MHC Class II (I-A/I-E) APC (right).](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0006.jpg)
![Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the Biotin conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with 0.015 ug of Rat IgG2b K Isotype Control Biotin (open histogram) or 0.015 ug of Anti-Mouse MHC Class II (I-A/I-E) Biotin followed by Streptavidin PE (filled histogram).](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0007.jpg)
![Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the FITC conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with Anti-Mouse CD3e and 0.06 micrograms of Rat IgG2b k Isotype Control FITC (left) or 0.06 micrograms of Anti-Mouse MHC Class II (I-A/I-E) FITC (right). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0008.jpg)
![TGF-beta signaling inhibits immunological characteristics of myofibers through activating the IRE1 alpha pathway. Western blot analyses of the expression changes of Smad pathway proteins, IRE1 alpha and PERK pathway proteins, and immunological molecules in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS and/or SRI (A); 24 or 48 h stimulation of IFN-gamma /LPS, SRI, 4-PBA, 4μ8C (IRE1 alpha pathway inhibitor) or GSK (PERK pathway inhibitor) (B, D). The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta , IL-6, and MCP-1 in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS, SRI and/or 4-PBA,analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-43312_rat-monoclonal-mhc-class-ii-i-a-i-e-antibody-m5-114-15-2-western-blot-13220261457494.jpg)
![Intrinsic TGF-beta signaling affects muscle cell immune behaviors by prompting UPR activity under pro-inflammatory conditions. The protein levels of IRE1 alpha and eIF2 alpha (A), H-2Kb, H2-E alpha , and TLR3 (B) in control or SM TGF-beta r2−/− mice-derived MPC-myotubes, with or without stimulation of IFN-gamma /LPS, Tg, or TM, were analyzed by Western blot. The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta , IL-6, and MCP-1 in MPC-myotubes with or without 48 h stimulation of IFN-gamma /LPS, Tg, or TM, analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-43312_rat-monoclonal-mhc-class-ii-i-a-i-e-antibody-m5-114-15-2-western-blot-13220261534476.jpg)
![TGF-beta signaling inhibits immunological characteristics of myofibers through activating the IRE1 alpha pathway. Western blot analyses of the expression changes of Smad pathway proteins, IRE1 alpha and PERK pathway proteins, and immunological molecules in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS and/or SRI (A); 24 or 48 h stimulation of IFN-gamma /LPS, SRI, 4-PBA, 4μ8C (IRE1 alpha pathway inhibitor) or GSK (PERK pathway inhibitor) (B, D). The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta , IL-6, and MCP-1 in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS, SRI and/or 4-PBA,analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-43312_rat-monoclonal-mhc-class-ii-i-a-i-e-antibody-m5-114-15-2-western-blot-132202615335818.jpg)
![TGF-beta signaling inhibits immunological characteristics of myofibers through activating the IRE1 alpha pathway. Western blot analyses of the expression changes of Smad pathway proteins, IRE1 alpha and PERK pathway proteins, and immunological molecules in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS and/or SRI (A); 24 or 48 h stimulation of IFN-gamma /LPS, SRI, 4-PBA, 4μ8C (IRE1 alpha pathway inhibitor) or GSK (PERK pathway inhibitor) (B, D). The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta , IL-6, and MCP-1 in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS, SRI and/or 4-PBA,analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-43312_rat-monoclonal-mhc-class-ii-i-a-i-e-antibody-m5-114-15-2-western-blot-1322026159715.jpg)
![Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-15365-img0027.jpg "<b>Independent Antibodies Validation. </b>Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Rat brain. alpha Synuclein antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Mouse hippocampus. alpha Synuclein stained by alpha Synuclein antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0033.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Human colon carcinoma, using alpha Synuclein antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0006.jpg)
![Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha-Synuclein antibody (NBP2-15365) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-91020242024611.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - alpha-Synuclein antibody detects alpha-Synuclein protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.alpha-Synuclein stained by alpha-Synuclein antibody (NBP2-15365) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-91020242021977.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - alpha-Synuclein antibody detects alpha-Synuclein protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.alpha-Synuclein stained by alpha-Synuclein antibody (NBP2-15365) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-91020242091454.jpg)
![alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:250.Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-immunohistochemistry-frozen-261120251834269.jpg)
![alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-immunocytochemistry-immunofluorescence-2611202518351723.jpg)
![alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-immunohistochemistry-frozen-261120251831548.jpg)
![Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0004.jpg "Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.")
![Simple Western: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Synuclein-alpha Antibody (2A7) [NBP1-05194] - Simple Western lane view shows a specific band for alpha-Synuclein in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Simple-Western-NBP1-05194-img0003.jpg)
![Western Blot: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Western blot analysis of tissue lysates using mAb to alpha-synuclein NBP1-05194. dilution 1:1,000 in green. Lane [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. Strong band at about 15kDa corresponds to alpha-synuclein protein.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Western-Blot-NBP1-05194-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody (2A7) [NBP1-05194] - Mixed neuron-glial cultures stained with NBP1-05194, our monoclonal antibody to alpha-synuclein (red) and chicken polylclonal antibody to MAP2 NB300-213 (green). The alpha-synuclein antibody stains vesicular structures the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0002.jpg)
![Flow Cytometry: alpha-Synuclein Antibody (2A7) [NBP1-05194] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NBP1-05194AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Flow-Cytometry-NBP1-05194-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human Synuclein alpha/beta Antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NBP1-22957-img0003.jpg "Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human Synuclein alpha/beta Antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).")
![Western Blot: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - The recombinant human synuclein family (alpha-,beta- and gamma-) (each 20ng), Mouse brain and Rat brain (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha,beta-synuclein (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Western-Blot-NBP1-22957-img0002.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of alpha, beta-Synuclein in C6 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0006.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0004.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of alpha, beta-Synuclein in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0005.jpg)
![Western Blot: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] - Western blot analysis of lysates from U-87MG cells, using Synuclein alpha/beta Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-33536_rabbit-synuclein-alpha-beta-mab-9e9x5-1012202411353043.jpg "Western Blot: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] - Western blot analysis of lysates from U-87MG cells, using Synuclein alpha/beta Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 3min.")
![Immunocytochemistry/ Immunofluorescence: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] - Immunofluorescence analysis of paraffin-embedded rat brain using Synuclein alpha/beta Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33536_rabbit-synuclein-alpha-beta-mab-9e9x5-1012202411334048.jpg)
![Western Blot: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] - Western blot analysis of various lysates using Synuclein alpha/beta Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1min.](http://images.novusbio.com/fullsize/nbp3-33536_rabbit-synuclein-alpha-beta-mab-9e9x5-101220241136068.jpg)
![Western Blot: Synuclein alpha/beta Antibody (SR1281) [NBP3-21662] - Western blot analysis using NBP3-21662 in Mouse brain tissue lysate.](http://images.novusbio.com/fullsize/nbp3-21662_rabbit-synuclein-alpha-beta-mab-sr1281-5720231595518.jpg "Western Blot: Synuclein alpha/beta Antibody (SR1281) [NBP3-21662] - Western blot analysis using NBP3-21662 in Mouse brain tissue lysate.")
![Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunofluorescence-NBP2-25146-img0010.jpg "Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.")
![Western Blot: alpha-Synuclein Antibody [NBP2-25146] - Western blot analysis of different tissue lysates using chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-25146-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Mixed rat neuron-glial cultures stained with NBP2-25146, polyclonal antibody to alpha-Synuclein (red) and monoclonal antibody to MAP2 (NBP2-25156, green). The alpha-Synuclein antibody stains vesicular structures, the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-Synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody [NBP2-25146] - Human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken-594 (1:1000) secondary antibody. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Image from verified customer review.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0006.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Western-Blot-NBP2-54789-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54789-img0004.jpg)
![Immunohistochemistry: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Immunohistochemistry analysis of rat brain injected with Type 1 human alpha synuclein PFFs (NBP2-54789). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 human alpha synuclein PFFs (NBP2-54789) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the striatum close to an injection site.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-54789-img0007.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to active alpha Synuclein aggregate (NBP2-54789) and active alpha Synuclein monomer (NBP2-54788) alone. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-54789-img0006.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0008.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1) (Catalog No. NBP2-54789). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Product-Image-NBP2-54789-img0010.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0009.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Western-Blot-NBP2-54787-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde made from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54787-img0006.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to when 100 uM of active alpha Synuclein monomer (NBP2-54788) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787), or 100 uM of control alpha Synuclein monomer (NBP2-54786) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787). Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-In-vitro-assay-NBP2-54787-img0007.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) (Catalog No. NBP2-54787) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Product-Image-NBP2-54787-img0009.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - TEM of Type 2 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54787)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Electron-Microscopy-NBP2-54787-img0008.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0005.jpg "Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded human kidney tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] -Analysis of HeLa cells labeling Phospho-alpha Synuclein (S129) with Rabbit anti-Phospho-alpha Synuclein (S129) antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-alpha Synuclein (S129) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75409_rabbit-monoclonal-alpha-synuclein-antibody-jb22-44-immunocytochemistry-immunofluorescence-1820247526..jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] -Analysis of Neuro-2a cells labeling Phospho-alpha Synuclein with Rabbit anti-Phospho-alpha Synuclein antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-alpha Synuclein antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75409_rabbit-monoclonal-alpha-synuclein-antibody-jb22-44-immunocytochemistry-immunofluorescence-18202475444..jpg)
![Flow Cytometry:alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of HeLa cells labeling Phospho-alpha Synuclein (S129).Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-75409_rabbit-monoclonal-alpha-synuclein-antibody-jb22-44-flow-cytometry-18202475655..jpg)