| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43312-0.5mg | |||
| BSA Free | NBP1-43312-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08856 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43312-0.5mg | |||
| BSA Free | NBP1-43312-0.1mg | |||
| BSA Free | NBP1-43312-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08856 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08856 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43312-0.5mg | |||
| BSA Free | NBP1-43312-0.1mg | |||
| BSA Free | NBP1-43312-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05194-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21662-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25146SS | |||
![Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the PE conjugate of NBP1-43312. Analysis of mouse splenocyte suspensions using MHC Class II PE and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0009.jpg "Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the PE conjugate of NBP1-43312. Analysis of mouse splenocyte suspensions using MHC Class II PE and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.")
![Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Staining of C57BL/6 splenocytes with 0.06 ug of Rat IgG2b K Isotype Control Purified (cat. NBP1-43323) (open histogram) or 0.06 ug of Anti-Mouse MHC Class II (I-A/I-E) Purified (filled histogram) followed by Anti-Rat IgG FITC (cat. NBP1-69751). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0001.jpg)
![Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the Allophycocyanin conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC and 0.015 micrograms of Rat IgG2b ? Isotype Control APC (left) or 0.015 micrograms of Anti-Mouse MHC Class II (I-A/I-E) APC (right).](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0006.jpg)
![Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the Biotin conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with 0.015 ug of Rat IgG2b K Isotype Control Biotin (open histogram) or 0.015 ug of Anti-Mouse MHC Class II (I-A/I-E) Biotin followed by Streptavidin PE (filled histogram).](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0007.jpg)
![Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the FITC conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with Anti-Mouse CD3e and 0.06 micrograms of Rat IgG2b k Isotype Control FITC (left) or 0.06 micrograms of Anti-Mouse MHC Class II (I-A/I-E) FITC (right). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0008.jpg)
![TGF-beta signaling inhibits immunological characteristics of myofibers through activating the IRE1 alpha pathway. Western blot analyses of the expression changes of Smad pathway proteins, IRE1 alpha and PERK pathway proteins, and immunological molecules in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS and/or SRI (A); 24 or 48 h stimulation of IFN-gamma /LPS, SRI, 4-PBA, 4μ8C (IRE1 alpha pathway inhibitor) or GSK (PERK pathway inhibitor) (B, D). The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta , IL-6, and MCP-1 in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS, SRI and/or 4-PBA,analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-43312_rat-monoclonal-mhc-class-ii-i-a-i-e-antibody-m5-114-15-2-western-blot-13220261457494.jpg)
![Intrinsic TGF-beta signaling affects muscle cell immune behaviors by prompting UPR activity under pro-inflammatory conditions. The protein levels of IRE1 alpha and eIF2 alpha (A), H-2Kb, H2-E alpha , and TLR3 (B) in control or SM TGF-beta r2−/− mice-derived MPC-myotubes, with or without stimulation of IFN-gamma /LPS, Tg, or TM, were analyzed by Western blot. The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta , IL-6, and MCP-1 in MPC-myotubes with or without 48 h stimulation of IFN-gamma /LPS, Tg, or TM, analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-43312_rat-monoclonal-mhc-class-ii-i-a-i-e-antibody-m5-114-15-2-western-blot-13220261534476.jpg)
![TGF-beta signaling inhibits immunological characteristics of myofibers through activating the IRE1 alpha pathway. Western blot analyses of the expression changes of Smad pathway proteins, IRE1 alpha and PERK pathway proteins, and immunological molecules in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS and/or SRI (A); 24 or 48 h stimulation of IFN-gamma /LPS, SRI, 4-PBA, 4μ8C (IRE1 alpha pathway inhibitor) or GSK (PERK pathway inhibitor) (B, D). The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta , IL-6, and MCP-1 in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS, SRI and/or 4-PBA,analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-43312_rat-monoclonal-mhc-class-ii-i-a-i-e-antibody-m5-114-15-2-western-blot-132202615335818.jpg)
![TGF-beta signaling inhibits immunological characteristics of myofibers through activating the IRE1 alpha pathway. Western blot analyses of the expression changes of Smad pathway proteins, IRE1 alpha and PERK pathway proteins, and immunological molecules in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS and/or SRI (A); 24 or 48 h stimulation of IFN-gamma /LPS, SRI, 4-PBA, 4μ8C (IRE1 alpha pathway inhibitor) or GSK (PERK pathway inhibitor) (B, D). The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta , IL-6, and MCP-1 in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS, SRI and/or 4-PBA,analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-43312_rat-monoclonal-mhc-class-ii-i-a-i-e-antibody-m5-114-15-2-western-blot-1322026159715.jpg)
![MHC class II (I-A/I-E) Antibody (MK-D6) [Alexa Fluor® 350] [NBP3-08856AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.](http://images.novusbio.com/fullsize/nbp3-08856af350_mouse-monoclonal-mhc-class-ii-i-a-i-e-antibody-mk-d6-alexa-fluor-350-29820239331795.jpg "MHC class II (I-A/I-E) Antibody (MK-D6) [Alexa Fluor® 350] [NBP3-08856AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.")
![Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-15365-img0027.jpg "<b>Independent Antibodies Validation. </b>Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Rat brain. alpha Synuclein antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Mouse hippocampus. alpha Synuclein stained by alpha Synuclein antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0033.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Human colon carcinoma, using alpha Synuclein antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0006.jpg)
![Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha-Synuclein antibody (NBP2-15365) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-91020242024611.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - alpha-Synuclein antibody detects alpha-Synuclein protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.alpha-Synuclein stained by alpha-Synuclein antibody (NBP2-15365) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-91020242021977.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - alpha-Synuclein antibody detects alpha-Synuclein protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.alpha-Synuclein stained by alpha-Synuclein antibody (NBP2-15365) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-91020242091454.jpg)
![alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:250.Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-immunohistochemistry-frozen-261120251834269.jpg)
![alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-immunocytochemistry-immunofluorescence-2611202518351723.jpg)
![alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-immunohistochemistry-frozen-261120251831548.jpg)
![Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0004.jpg "Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.")
![Simple Western: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Synuclein-alpha Antibody (2A7) [NBP1-05194] - Simple Western lane view shows a specific band for alpha-Synuclein in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Simple-Western-NBP1-05194-img0003.jpg)
![Western Blot: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Western blot analysis of tissue lysates using mAb to alpha-synuclein NBP1-05194. dilution 1:1,000 in green. Lane [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. Strong band at about 15kDa corresponds to alpha-synuclein protein.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Western-Blot-NBP1-05194-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody (2A7) [NBP1-05194] - Mixed neuron-glial cultures stained with NBP1-05194, our monoclonal antibody to alpha-synuclein (red) and chicken polylclonal antibody to MAP2 NB300-213 (green). The alpha-synuclein antibody stains vesicular structures the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0002.jpg)
![Flow Cytometry: alpha-Synuclein Antibody (2A7) [NBP1-05194] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NBP1-05194AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Flow-Cytometry-NBP1-05194-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human Synuclein alpha/beta Antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NBP1-22957-img0003.jpg "Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human Synuclein alpha/beta Antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).")
![Western Blot: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - The recombinant human synuclein family (alpha-,beta- and gamma-) (each 20ng), Mouse brain and Rat brain (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha,beta-synuclein (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Western-Blot-NBP1-22957-img0002.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of alpha, beta-Synuclein in C6 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0006.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0004.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of alpha, beta-Synuclein in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0005.jpg)
![Western Blot: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] - Western blot analysis of lysates from U-87MG cells, using Synuclein alpha/beta Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-33536_rabbit-synuclein-alpha-beta-mab-9e9x5-1012202411353043.jpg "Western Blot: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] - Western blot analysis of lysates from U-87MG cells, using Synuclein alpha/beta Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 3min.")
![Immunocytochemistry/ Immunofluorescence: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] - Immunofluorescence analysis of paraffin-embedded rat brain using Synuclein alpha/beta Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33536_rabbit-synuclein-alpha-beta-mab-9e9x5-1012202411334048.jpg)
![Western Blot: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] - Western blot analysis of various lysates using Synuclein alpha/beta Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1min.](http://images.novusbio.com/fullsize/nbp3-33536_rabbit-synuclein-alpha-beta-mab-9e9x5-101220241136068.jpg)
![Western Blot: Synuclein alpha/beta Antibody (SR1281) [NBP3-21662] - Western blot analysis using NBP3-21662 in Mouse brain tissue lysate.](http://images.novusbio.com/fullsize/nbp3-21662_rabbit-synuclein-alpha-beta-mab-sr1281-5720231595518.jpg "Western Blot: Synuclein alpha/beta Antibody (SR1281) [NBP3-21662] - Western blot analysis using NBP3-21662 in Mouse brain tissue lysate.")
![Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunofluorescence-NBP2-25146-img0010.jpg "Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.")
![Western Blot: alpha-Synuclein Antibody [NBP2-25146] - Western blot analysis of different tissue lysates using chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-25146-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Mixed rat neuron-glial cultures stained with NBP2-25146, polyclonal antibody to alpha-Synuclein (red) and monoclonal antibody to MAP2 (NBP2-25156, green). The alpha-Synuclein antibody stains vesicular structures, the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-Synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody [NBP2-25146] - Human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken-594 (1:1000) secondary antibody. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Image from verified customer review.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0006.jpg)