EMR2 Antibody (494025) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
494025
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

EMR2 Antibody (494025) [Alexa Fluor® 750] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human EMR2
Gln24-Asn823
Accession # AAI27006
Specificity
Detects human EMR2 in EMR2-transfected cells but not in the parental cell line.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EMR2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EMR2 Antibody (494025) [Alexa Fluor® 750]

  • CD312
  • DKFZp781B135
  • egf-like module containing, mucin-like, hormone receptor-like 2
  • egf-like module containing, mucin-like, hormone receptor-like sequence 2
  • EGF-like module receptor 2
  • EGF-like module-containing mucin-like hormone receptor-like 2
  • EMR2

Background

EMR2 (EGF-like module-containing mucin-like receptor 2; designated CD312) is a glycoprotein belonging to the EGF-TM7 family of adhesion-type class B 7‑transmembrane (TM) receptors. EGF-like sequences within long extracellular N-termini, and a GPS (G-protein proteolytic site) domain are characteristic of this family, which is mainly expressed on cells of the immune system (1, 2). The human EMR2 cDNA encodes an 823 amino acid (aa) protein with five EGF-like domains within the first 250 aa, followed by a mucin-like stalk, a GPS domain (aa 479‑530) and a 7‑TM sequence (aa 531‑785). The GPS domain is the site of autocatalytic cleavage, forming two cleaved portions that remain non-covalently attached as a heterodimer (1, 3). Of the first 290 aa of human EMR2, 284 aa (97%) are identical with family member CD97, likely due to gene duplication (2). The portion of human EMR2 N-terminal to the GPS domain (aa 1‑478) shares 64%, 59%, 48% and 45% aa identity with corresponding regions of canine EMR2, equine EMR2, mouse CD97 and rat CD97, respectively. Alternate splicing of EMR2 creates isoforms that contain 2‑5 EGF-like domains. Only the 5-EGF form contains EGF4, which is necessary for calcium-dependent binding of the EMR2/CD97 ligand, chondroitin sulfate (CS) (2, 4‑6). None of the isoforms engage the CD97 ligand, CD55 (DAF). EMR2 is restricted to myeloid cells (1, 2). EMR2 expression increases as monocytes differentiate into macrophages, and decreases with differentiation into dendritic cells (5). Activation increases neutrophil EMR2 expression (5). EMR2 localizes to the leading edge of migrating neutrophils and plays an important role in migration, adhesion and superoxide production (7). It is also thought to facilitate specific interaction of myeloid cells with peripheral B lymphocytes which express CS (6).

  1. Kwakkenbos, M.J. et al. (2004) Immunogenetics 55:655.
  2. Lin, H.-H. et al. (2000) Genomics 67:188.
  3. Lin, H.-H. et al. (2004) J. Biol. Chem. 279:31823.
  4. Stacey, M. et al. (2003) Blood 102:2916.
  5. Chang G.-W. et al. (2007) Biochem. Biophys. Res. Commun. 353:133.
  6. Kwakkenbos, M.J. et al. (2005) J. Leukoc. Biol. 77:112.
  7. Yona, S. et al. (2008) FASEB J. 22:741.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EMR2
Entrez
Uniprot