Dkk-1 Antibodies

HAF017). Specific bands were detected for Dkk-1 at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for Dkk-1 at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Dkk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Dkk-1 at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). Specific bands were detected for Dkk-1 at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for Dkk-1 at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for Dkk-1 at approximately 35 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for Dkk-1 at approximately 35 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

5439-DK) was serially diluted and captured by Mouse Anti-Human Dkk‑1 Monoclonal Antibody (Catalog # MAB10962) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 5439-DK) was serially diluted and captured by Mouse Anti-Human Dkk‑1 Monoclonal Antibody (Catalog # MAB10962) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Dkk‑1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye) using Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

1765-DK) was serially diluted and captured by Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1765-DK) was serially diluted and captured by Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1765-DK) was serially diluted and captured by Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1765-DK) was serially diluted and captured by Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Dkk-1 was detected in immersion fixed paraffin-embedded sections of human colon using Human Dkk-1 Monoclonal Antibody (Catalog # MAB10962) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

5439-DK) was serially diluted and captured by Mouse Anti-Human Dkk‑1 Monoclonal Antibody (Catalog # MAB10962) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 5439-DK) was serially diluted and captured by Mouse Anti-Human Dkk‑1 Monoclonal Antibody (Catalog # MAB10962) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in lung. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in lung. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Dkk‑1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c) using Mouse Dkk‑1 Monoclonal Antibody (Catalog # MAB1765) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in lung. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />