CXCL8/IL-8 Antibodies

HAF018). A specific band was detected for IL-8/CXCL8 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IL-8/CXCL8 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 3 hours. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB2083) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IL-8/CXCL8 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

2277-FL) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB2277). The ND₅₀ is typically 0.08-0.32 µg/mL." class="big_lightbox"> 2277-FL) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB2277). The ND₅₀ is typically 0.08-0.32 µg/mL." alt="Recombinant Feline CXCL8/IL-8 (Catalog # 2277-FL) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB2277). The ND₅₀ is typically 0.08-0.32 µg/mL." class="big_thumb" />

MAB2081) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB2082) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> MAB2081) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB2082) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human IL-8/CXCL8 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (MAB2081) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB2082) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

DY990). Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (MAB2082) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY9998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (MAB2082) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY9998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human IL-8/CXCL8 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB2081) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (MAB2082) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY9998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />