| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10915-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB120-6652 | |||
| FITC | NBP1-97372 | |||
| HRP | NB120-6651 | |||
| PE | NBP1-97335 | |||
| Rhodamine | NBP1-97383-0.5mg | |||
| Texas Red | NB110-3647 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-27043 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20861 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 405 | NBP3-50139AFP405 | |||
| Alexa Fluor Plus 488 | NBP3-50139AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-50139AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-50139AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-50139AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-50139AFP680 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 405 | NBP3-50138AFP405 | |||
| Alexa Fluor Plus 488 | NBP3-50138AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-50138AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-50138AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-50138AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-50138AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15606-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50120-0.025ml | |||
![Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Texas Red conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Biotin-Antibody-Western-Blot-NB120-6643-img0005.jpg "Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Texas Red conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.")
![Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Fluorescein conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Biotin-Antibody-Western-Blot-NB120-6643-img0004.jpg)
![ELISA: Biotin Antibody [NB120-6643] - Analysis using BIOTIN Antibody DyLight 488.](http://images.novusbio.com/fullsize/Biotin-Antibody-ELISA-NB120-6643-img0001.jpg)
![ELISA: Biotin Antibody [NB120-6643] - ELISA results of purified Goat anti-Biotin Antibody Peroxidase Conjugated tested against purified biotin. Each well was coated in duplicate with 1.0 ug of Bovine Albumin Biotin Conjugated. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3 percent fish gelatin as blocking buffer and TMB substrate. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Biotin Antibody-ELISA-NB120-6643-img0007.jpg)
![Western Blot: Biotin Antibody [NBP2-15589] - Western blot experiment using at 1:90000 dilution to detect 60ng of biotinylated protein and non-biotinylated control. A: Non-biotinlated control. B: Biotinylated protein.](http://images.novusbio.com/fullsize/Biotin-Antibody-Western-Blot-NBP2-15589-img0001.jpg "Western Blot: Biotin Antibody [NBP2-15589] - Western blot experiment using at 1:90000 dilution to detect 60ng of biotinylated protein and non-biotinylated control. A: Non-biotinlated control. B: Biotinylated protein.")
![Western Blot: Biotin Antibody - Azide and BSA Free [Biotin] - Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 100 ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15606_rabbit-biotin-pab-5220251734516.jpg "Western Blot: Biotin Antibody - Azide and BSA Free [Biotin] - Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 100 ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.")
![Western Blot: Biotin Antibody (7H10R8) [NBP3-16062] - Western blot analysis of various lysates, using Biotin Rabbit mAb at 1:300 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16062_rabbit-biotin-mab-7h10r8-522025125429.jpg "Western Blot: Biotin Antibody (7H10R8) [NBP3-16062] - Western blot analysis of various lysates, using Biotin Rabbit mAb at 1:300 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![ELISA: Biotin Antibody (1D4-C5) [NBP3-23510] - ELISA analysis of a biotinylated antigen using biotin-specific antibody and streptavidin. Microtitration wells were coated with biotinylated human IgG (0.01 ug/ml) and blocked. Then either anti-biotin mouse monoclonal 1D4-C5 (A, C) or streptavidin-HRP (B) was added in six different doses. Finally, goat anti-mouse-HRP conjugate (A) or streptavidin-HRP (C) was added. HRP signal increased in dose dependent manner, but with different dynamics, when biotinylated antibody was detected by anti-biotin mAb/goat anti mouse-HRP (A) or by streptavidin-HRP (B), and it was falling to zero in dose dependent manner when streptavidin-HRP was competed by anti-biotin mAb 1D4-C5 (C).](http://images.novusbio.com/fullsize/nbp3-23510_mouse-biotin-mab-1d4-c5-792023121223.jpg "ELISA: Biotin Antibody (1D4-C5) [NBP3-23510] - ELISA analysis of a biotinylated antigen using biotin-specific antibody and streptavidin. Microtitration wells were coated with biotinylated human IgG (0.01 ug/ml) and blocked. Then either anti-biotin mouse monoclonal 1D4-C5 (A, C) or streptavidin-HRP (B) was added in six different doses. Finally, goat anti-mouse-HRP conjugate (A) or streptavidin-HRP (C) was added. HRP signal increased in dose dependent manner, but with different dynamics, when biotinylated antibody was detected by anti-biotin mAb/goat anti mouse-HRP (A) or by streptavidin-HRP (B), and it was falling to zero in dose dependent manner when streptavidin-HRP was competed by anti-biotin mAb 1D4-C5 (C).")