Biotin Antibody (1D4-C5) - BSA Free

ELISA: Biotin Antibody (1D4-C5) [NBP3-23510] - ELISA analysis of a biotinylated antigen using biotin-specific antibody and streptavidin. Microtitration wells were coated with biotinylated human IgG (0.01 ug/ml) and blocked. Then either anti-biotin mouse monoclonal 1D4-C5 (A, C) or streptavidin-HRP (B) was added in six different doses. Finally, goat anti-mouse-HRP conjugate (A) or streptavidin-HRP (C) was added. HRP signal increased in dose dependent manner, but with different dynamics, when biotinylated antibody was detected by anti-biotin mAb/goat anti mouse-HRP (A) or by streptavidin-HRP (B), and it was falling to zero in dose dependent manner when streptavidin-HRP was competed by anti-biotin mAb 1D4-C5 (C).

• Reactivity: All-NA
• Applications: ELISA, Flow, IP, CyTOF-ready
• Clone: 1D4-C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Biotin Antibody (1D4-C5) - BSA Free Summary

• Description: Novus Biologicals Mouse Biotin Antibody (1D4-C5) - BSA Free (NBP3-23510) is a monoclonal antibody validated for use in ELISA, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Biotinylated Limulus hemocyanin
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Biotin Antibody (1D4-C5) - BSA Free

• Biotin
• Vitamin B4

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.