B7-H4 Antibodies
We offer B7-H4 Antibodies for use in common research applications: Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each B7-H4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our B7-H4 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the B7-H4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our B7-H4 polyclonal antibodies and browse our B7-H4 monoclonal antibody catalog.
Alternate Names for B7-H4 Antibodies
anti-B7-H4 antibody, anti-VTCN1 antibody, anti-B7h.5 antibody, anti-B7H4T-cell costimulatory molecule B7x antibody, anti-B7S1VCTN1 antibody, anti-B7XPRO1291 antibody, anti-FLJ22418 antibody, anti-Immune costimulatory protein B7-H4 antibody, anti-Protein B7S1 antibody, anti-T cell costimulatory molecule B7x antibody, anti-V-set domain containing T cell activation inhibitor 1 antibody, anti-V-set domain-containing T-cell activation inhibitor 1 antibody
25 Results for "b7-h4" in Primary Antibodies
![Western Blot: B7-H4 Antibody (PSH03-65) [NBP3-32065] - Western blot analysis of B7-H4 on different lysates with Rabbit anti-B7-H4 antibody (NBP3-32065) at 1/1,000 dilution. Lane 1: MDA-MB-468 cell lysate (no heat) (15 ug/Lane) Lane 2: HeLa cell lysate (negative) (30 ug/Lane) Lane 3: NIH:OVCAR-3 cell lysate (30 ug/Lane) Lane 4: Mouse uterus tissue lysate (30 ug/Lane) Lane 5: Rat ovary tissue lysate (15 ug/Lane) Predicted band size: 31 kDa Observed band size: 60-75 kDa Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32065) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32065_rabbit-b7-h4-mab-psh03-65-278202416331223.jpg "Western Blot: B7-H4 Antibody (PSH03-65) [NBP3-32065] - Western blot analysis of B7-H4 on different lysates with Rabbit anti-B7-H4 antibody (NBP3-32065) at 1/1,000 dilution. Lane 1: MDA-MB-468 cell lysate (no heat) (15 ug/Lane) Lane 2: HeLa cell lysate (negative) (30 ug/Lane) Lane 3: NIH:OVCAR-3 cell lysate (30 ug/Lane) Lane 4: Mouse uterus tissue lysate (30 ug/Lane) Lane 5: Rat ovary tissue lysate (15 ug/Lane) Predicted band size: 31 kDa Observed band size: 60-75 kDa Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32065) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: B7-H4 Antibody (PSH03-65) [NBP3-32065] - Immunocytochemistry analysis of SK-Br-3 (positive) and HeLa (negative) labeling B7-H4 with Rabbit anti-B7-H4 antibody (NBP3-32065) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-B7-H4 antibody (NBP3-32065) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32065_rabbit-b7-h4-mab-psh03-65-2920241192329.jpg)
![Flow Cytometry: B7-H4 Antibody (188) [NBP1-43266] - Staining of non-transfected (left) and mouse B7-H4 transfected (right) L5178Y cells with 0.25 ug Rat IgG2b kappa Isotype Control Purified (open histogram) or 0.25 ug Anti-Mouse B7-H4 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/B7-H4-Antibody-188-Flow-Cytometry-NBP1-43266-img0002.jpg "Flow Cytometry: B7-H4 Antibody (188) [NBP1-43266] - Staining of non-transfected (left) and mouse B7-H4 transfected (right) L5178Y cells with 0.25 ug Rat IgG2b kappa Isotype Control Purified (open histogram) or 0.25 ug Anti-Mouse B7-H4 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Total viable cells were used for analysis.")
