HAF008). A specific band was detected for B7‑H3 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of C2C12 mouse myoblast cell line and MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse B7‑H3 Monoclonal Antibody (Catalog # MAB1397) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for B7‑H3 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1397-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75195-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75194-0.025mg | |||
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB Counterstain: IHC hematoxylin](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP2-32251-img0002.jpg "Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB Counterstain: IHC hematoxylin")
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Samples: FFPE sections of MCF-7 cells (endogenous expression) and CD276 transfected Hek293 cells (over-expression). Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunocytochemistry-NBP2-32251-img0003.jpg)
![Immunoprecipitation: B7-H3/CD276 Antibody [NBP2-32251] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-CD276 antibody NBP2-32251 used for IP at 6 ug per reaction. CD276 was also immunoprecipitated by rabbit anti-CD276 antibody BL16383. For blotting immunoprecipitated CD276 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunoprecipitation-NBP2-32251-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Immunocytochemistry analysis of THP-1 cells labeling B7-H3/CD276 with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32139_rabbit-b7-h3-cd276-mab-psh02-46-2920241048482.jpg "Immunocytochemistry/ Immunofluorescence: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Immunocytochemistry analysis of THP-1 cells labeling B7-H3/CD276 with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Flow cytometric analysis of THP-1 cells labeling B7-H3/CD276.<br/><br/> Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32139_rabbit-b7-h3-cd276-mab-psh02-46-392024884623.jpg)
![Western blot analysis on different lysates with NBP3-32139 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: LoVo cell lysate Lane 5: U-2 OS cell lysate Lane 6: LNCaP cell lysate Lane 7: SH-SY5Y cell lysate Lane 8: THP-1 cell lysate Lane 9: HCT 116 cell lysate Lane 10: Raji cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32139 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32139_-western-blot-639204597140646475.jpg)
![Flow Cytometry: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (NBP2-75195) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75195, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-MJ18-Chimeric-Flow-Cytometry-NBP2-75195-img0002.jpg "Flow Cytometry: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (NBP2-75195) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75195, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody")
![ELISA: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Binding curve of anti-B7-H3 antibody MJ18 (NBP2-75195) to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-75195. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-MJ18-Chimeric-ELISA-NBP2-75195-img0001.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (RM335) [NBP2-77415] - Staining of human colon cancer tissue section using NBP2-77415.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-RM335-Immunohistochemistry-NBP2-77415-img0001.jpg "Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (RM335) [NBP2-77415] - Staining of human colon cancer tissue section using NBP2-77415.")
![Western Blot: B7-H3/CD276 Antibody (RM335) [NBP2-77415] - Western Blot of LNCaP cell lysate using Clone RM335 at a 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-77415_rabbit-monoclonal-b7-h3-cd276-antibody-rm335-24620241245910.png)
![Western Blot: B7-H3/CD276 Antibody (MM0104-20J12) [NBP2-12048] - Lysates of human gastric cancer cells](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-MM0104-20J12-Western-Blot-NBP2-12048-img0001.jpg "Western Blot: B7-H3/CD276 Antibody (MM0104-20J12) [NBP2-12048] - Lysates of human gastric cancer cells")
