| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57331-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89670-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89669-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38514-20ul | |||
![Western Blot: THOC5 Antibody [NBP1-19160] - Association of NCBP3 and NCBP2 with NCBP1 and proteins involved in RNA processing. Western blot analysis of representative GFP-precipitated proteins identified by AP-MS analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms9192), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/THOC5-Antibody-Western-Blot-NBP1-19160-img0008.jpg "Western Blot: THOC5 Antibody [NBP1-19160] - Association of NCBP3 and NCBP2 with NCBP1 and proteins involved in RNA processing. Western blot analysis of representative GFP-precipitated proteins identified by AP-MS analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms9192), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: THOC5 Antibody [NBP1-19160] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- THOC5 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/THOC5-Antibody-Immunohistochemistry-NBP1-19160-img0007.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: THOC5 Antibody [NBP1-19160] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. THOC5 was also immunoprecipitated by rabbit anti-THOC5 antibody NBP1-19161.](http://images.novusbio.com/fullsize/THOC5-Antibody-Western-Blot-NBP1-19160-img0003.jpg)
![Immunohistochemistry: THOC5 Antibody - BSA Free [NBP3-45247] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (THOC5 Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-45247_rabbit-thoc5-pab-bsa-free-28420251258799.jpg "Immunohistochemistry: THOC5 Antibody - BSA Free [NBP3-45247] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (THOC5 Antibody) at dilution of 1:50")
![Western Blot: THOC5 Antibody - BSA Free [NBP3-45247] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (THOC5 Antibody) at dilution of 1:600](http://images.novusbio.com/fullsize/nbp3-45247_rabbit-thoc5-pab-bsa-free-28420251363794.jpg)
![Immunoprecipitation: THOC5 Antibody - BSA Free [NBP3-45247] - IP Result of anti-THOC5 (IP, 3ug; Detection: 1:300) with HEK-293 cells lysate 2000ug.](http://images.novusbio.com/fullsize/nbp3-45247_rabbit-thoc5-pab-bsa-free-28420251258788.jpg)
![Western Blot: THOC5 Antibody [NBP2-20621] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/THOC5-Antibody-Western-Blot-NBP2-20621-img0001.jpg "Western Blot: THOC5 Antibody [NBP2-20621] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: THOC5 Antibody [NBP2-20621] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/THOC5-Antibody-Immunohistochemistry-Paraffin-NBP2-20621-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: THOC5 Antibody [NBP2-57331] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/THOC5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57331-img0001.jpg "Immunocytochemistry/Immunofluorescence: THOC5 Antibody [NBP2-57331] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunocytochemistry-Immunofluorescence-NB100-174-img0017.jpg "Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.")
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunohistochemistry-Paraffin-NB100-174-img0019.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0008.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0010.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0011.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 western in 293T cells. Image from verified customer review.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0012.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0013.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Nuclear Matrix Protein p84 stained by hHpr1-p84-Thoc1 antibody [5E10] (NB100-174). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunohistochemistry-Paraffin-NB100-174-img0015.jpg)
![Immunoprecipitation: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 ug of p84 antibody [5E10] (NB100-174) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (NB100-174). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunoprecipitation-NB100-174-img0016.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr1-p84-Thoc1 antibody [5E10] (NB100-174) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb100-174_mouse-monoclonal-hhpr1-p84-thoc1-antibody-5e10-western-blot-1411202518352219.jpg)
![Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human cell line U-251 MG shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89670-img0011.jpg "Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human cell line U-251 MG shows localization to nuclear speckles.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human cerebral cortex, colon, skeletal muscle and testis using Anti-THOC1 antibody NBP1-89670 (A) shows similar protein distribution across tissues to independent antibody NBP1-89669 (B).](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0013.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0009.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0008.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human colon.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0015.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0016.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human skeletal muscle using Anti-THOC1 antibody NBP1-89670.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Analysis using Anti-THOC1 antibody NBP1-89670 (A) shows similar pattern to independent antibody NBP1-89669 (B).](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP1-89670-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89669-img0007.jpg "Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human cerebral cortex, colon, skeletal muscle and testis using Anti-THOC1 antibody NBP1-89669 (A) shows similar protein distribution across tissues to independent antibody NBP1-89670 (B).](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining in human appendix and skeletal muscle tissues using anti-THOC1 antibody. Corresponding THOC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0010.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0008.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0009.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human skeletal muscle using Anti-THOC1 antibody NBP1-89669.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0016.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human testis.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0013.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0014.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human colon.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Analysis using Anti-THOC1 antibody NBP1-89669 (A) shows similar pattern to independent antibody NBP1-89670 (B).](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP1-89669-img0012.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr-p84-Thoc1 Antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP2-16822-img0011.jpg "Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr-p84-Thoc1 Antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP2-16822-img0002.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP2-16822-img0004.jpg)
![Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of p84 by 3 ug of Nuclear Matrix Protein p84 antibody [C1C3] 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunoprecipitation-NBP2-16822-img0006.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] (NBP2-16822) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16822_rabbit-polyclonal-hhpr1-p84-thoc1-antibody-western-blot-2611202518274610.jpg)
![Nuclear Matrix Protein p84 antibody [C1C3] detects Nuclear Matrix Protein p84 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nuclear Matrix Protein p84 protein stained by Nuclear Matrix Protein p84 antibody [C1C3] (NBP2-16822) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16822_rabbit-polyclonal-hhpr1-p84-thoc1-antibody-immunocytochemistry-immunofluorescence-261120251848236.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Western blot analysis of various lysates using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-101220241224183.jpg "Western Blot: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Western blot analysis of various lysates using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Immunofluorescence analysis of U2OS cells using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-1012202411552836.jpg)
![Immunohistochemistry: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-1012202411592870.jpg)
![Immunohistochemistry: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-10122024120613.jpg)
![Immunohistochemistry: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-101220241224156.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Western blot analysis of various lysates using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-1012202411545561.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - Detection of Human THOC1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THOC1 antibody NBP1-47304 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP1-47304-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - Detection of Human THOC1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THOC1 antibody NBP1-47304 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- THOC1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-NBP1-47304-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - Detection of human THOC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-THOC1 antibody NBP1-47304 used for IP at 3 ug per reaction. THOC1 was also immunoprecipitated by another rabbit anti-THOC1 antibody. For blotting immunoprecipitated THOC1, NBP1-47304 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunoprecipitation-NBP1-47304-img0006.jpg)