| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87772-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87771-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35916-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97596-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5976-SP | |||
HAF018). A specific band was detected for Nucleoporin NUP85 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NR8383 rat alveolar macrophage cell line, K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, M1 mouse myeloid leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat Nucleoporin NUP85 Monoclonal Antibody (Catalog # MAB5976) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nucleoporin NUP85 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5976-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31860-25ul | |||
![Western Blot: Pericentrin Antibody [NB100-61071] - Whole cell lysate (10 ug) from HEK293T, HeLa, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pericentrin-Kendrinantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 second.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Western-Blot-NB100-61071-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NB100-61071] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight (R) 594 Conjugated used at a dilution of 1:100. Counterstain. DAPI (blue) and Phalloidin (green)](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-61071-img0014.jpg)
![Immunoprecipitation: Pericentrin Antibody [NB100-61071] - Detection of human Pericentrin/Kendrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pericentrin/Kendrin antibody NB100-61071 (lot 3) used for IP at 6 ug per reaction. Pericentrin/Kendrin was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated Pericentrin/Kendrin, NB100-61071 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunoprecipitation-NB100-61071-img0015.jpg)
![Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex, liver, placenta and testis using Anti-Pericentrin antibody NBP1-87772 (A) shows similar protein distribution across tissues to independent antibody NBP1-87771 (B).](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0017.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex, liver, placenta and testis using Anti-Pericentrin antibody NBP1-87772 (A) shows similar protein distribution across tissues to independent antibody NBP1-87771 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0018.jpg)
![Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining in human testis and liver tissues using anti-PCNT antibody. Corresponding PCNT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0010.jpg)
![Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP1-87771] - Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87771-img0004.jpg "Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP1-87771] - Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.")
![Western Blot: Pericentrin Antibody [NBP3-35916] - Western blot analysis of various lysates using Pericentrin Rabbit pAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35916_rabbit-pericentrin-pab-912202415161820.jpg "Western Blot: Pericentrin Antibody [NBP3-35916] - Western blot analysis of various lysates using Pericentrin Rabbit pAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NBP3-35916] - Immunofluorescence analysis of U-2 OS cells using Pericentrin Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35916_rabbit-pericentrin-pab-9122024159538.jpg)
![Western Blot: Pericentrin Antibody [NB100-63208] - Staining of HeLa lysate (35 ug protein in RIPA buffer) using Goat anti Human pericentrin 1 antibody at 1.0 ug/mL. Primary incubation was 1 hour. Signal was detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Western-Blot-NB100-63208-img0001.jpg "Western Blot: Pericentrin Antibody [NB100-63208] - Staining of HeLa lysate (35 ug protein in RIPA buffer) using Goat anti Human pericentrin 1 antibody at 1.0 ug/mL. Primary incubation was 1 hour. Signal was detected by chemiluminescence.")
![Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody (PSH01-66) [NBP3-32737] - Immunocytochemistry analysis of HeLa cells labeling Pericentrin with Rabbit anti-Pericentrin antibody (NBP3-32737) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pericentrin antibody (NBP3-32737) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32737_rabbit-pericentrin-mab-psh01-66-29202410234213.jpg "Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody (PSH01-66) [NBP3-32737] - Immunocytochemistry analysis of HeLa cells labeling Pericentrin with Rabbit anti-Pericentrin antibody (NBP3-32737) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pericentrin antibody (NBP3-32737) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Western blot analysis on different lysates with NBP3-32737 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lane 4: 293T cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 378 kDa Observed band size: 460 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32737 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32737_-western-blot-639204595245329884.jpg)
![Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP2-97596] - Immunofluorescence staining of Pericentrin in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97596 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Centrosome.](http://images.novusbio.com/fullsize/nbp2-97596_rabbit-polyclonal-pericentrin-antibody-235202514492356.png "Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP2-97596] - Immunofluorescence staining of Pericentrin in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97596 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Centrosome.")
![Immunohistochemistry-Paraffin: Nucleoporin NUP85 Antibody [NBP2-31860] - Nucleoporin NUP85/Pericentrin 1 Antibody [NBP2-31860] - Staining in human testis and skeletal muscle tissues using anti-NUP85 antibody. Corresponding NUP85 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nucleoporin-NUP85-Pericentrin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-31860-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nucleoporin NUP85 Antibody [NBP2-31860] - Nucleoporin NUP85/Pericentrin 1 Antibody [NBP2-31860] - Staining in human testis and skeletal muscle tissues using anti-NUP85 antibody. Corresponding NUP85 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Nucleoporin NUP85/Pericentrin 1 Antibody [NBP2-31860] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10<br/>Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/Nucleoporin-NUP85-Pericentrin-1-Antibody-Western-Blot-NBP2-31860-img0010.jpg)
![Immunohistochemistry-Paraffin: Nucleoporin NUP85/Pericentrin 1 Antibody [NBP2-31860] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Nucleoporin-NUP85-Pericentrin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-31860-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleoporin NUP85/Pericentrin 1 Antibody [NBP2-31860] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Nucleoporin-NUP85-Pericentrin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-31860-img0008.jpg)
