CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="ECE-2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 3 µg/mL Rat Anti-Human ECE-2 Monoclonal Antibody (Catalog # MAB16451) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB16451-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1645-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81495-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56945-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05677-20ul | |||
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ECE-2 Antibody [NBP1-81495] - Staining in human adrenal gland and endometrium tissues using anti-ECE2 antibody. Corresponding ECE2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ECE-2-Antibody-Immunohistochemistry-Paraffin-NBP1-81495-img0007.jpg)
![Immunohistochemistry-Paraffin: ECE-2 Antibody [NBP1-81495] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/ECE-2-Antibody-Immunohistochemistry-Paraffin-NBP1-81495-img0005.jpg)
![Immunohistochemistry-Paraffin: ECE-2 Antibody [NBP1-81495] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/ECE-2-Antibody-Immunohistochemistry-Paraffin-NBP1-81495-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ECE-2 Antibody [NBP2-56945] - Staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/fullsize/ECE-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56945-img0001.jpg "Immunocytochemistry/Immunofluorescence: ECE-2 Antibody [NBP2-56945] - Staining of human cell line U-2 OS shows localization to nucleus.")
![Western Blot: ECE-2 Antibody [NBP3-05677] - Western blot analysis of extracts of various cell lines, using ECE-2 antibody (NBP3-05677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ECE-2 Antibody-Western Blot-NBP3-05677-img0001.jpg "Western Blot: ECE-2 Antibody [NBP3-05677] - Western blot analysis of extracts of various cell lines, using ECE-2 antibody (NBP3-05677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.")
![Western Blot: ECE2 Antibody [NBP1-69061] - Rat Brain lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/ECE2-Antibody-Western-Blot-NBP1-69061-img0002.jpg "Western Blot: ECE2 Antibody [NBP1-69061] - Rat Brain lysate, concentration 0.2-1 ug/ml.")
