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VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771). The ND50 is typically 0.2-1.0 µg/mL." class="big_lightbox" target="_blank">
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox" target="_blank">
201-LB) induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line),as measured by the Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-1 beta /IL-1F2 is neutralized (green line) by increasing concentrations of Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269). The ND50 is typically < 5 µg/mL in the presence of 400 pg/mL Recombinant Human IL-1 beta /IL-1F2." class="big_lightbox" target="_blank">
269-1R) was serially diluted and captured by Mouse Anti-Human IL‑1 RI Monoclonal Antibody (Catalog # MAB269) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
269-1R) was serially diluted and captured by Mouse Anti-Human IL‑1 RI Monoclonal Antibody (Catalog # MAB269) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
The Interleukin 1 receptor type I (IL-1R1) belongs to the IL-1 receptor family (1). IL-1R1 binds to IL-1 alpha, IL-1 beta, and interleukin-1 receptor antagonist protein (IL-1RA). IL-1RA regulates IL-1 ligand biological activity by a competitive interaction for IL-1R1. Upon IL-1 stimulation, adaptor molecule MyD88 is first recruited to the IL-1R1-IL1R accessory protein complex, followed by the recruitment of two serine-threonine kinases, IRAK4 and IRAK, and the adaptor protein TRAF6, resulting in activation of the NF-kB pathway (2-3). Stimulation of IL-1R1, leads to activation of the transcription factors NF-B, ATF, and AP-1.
![Immunohistochemistry IL-1 RI Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL1_RI_AF771_Immunohistochemistry_22597.jpg)
![Flow Cytometry IL-1 RI Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL-1_RI_AF269_Flow_Cytometry_9910.jpg)
![Sandwich ELISA IL-1 RI Antibody Pair [HRP]](http://images.novusbio.com/fullsize/IL-1-RI-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79665-img0001.jpg)
![Sandwich ELISA IL-1 RI Antibody Pair [HRP]](http://images.novusbio.com/fullsize/IL-1-RI-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79625-img0001.jpg)
![Sandwich ELISA IL-1 RI Antibody Pair [HRP]](http://images.novusbio.com/fullsize/IL-1-RI-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79604-img0001.jpg)
![N/A IL-1 RI [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL1_RI_DY269_ELISA_1875.jpg)
![N/A IL-1 RI [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL1_RI_DY77105_ELISA_2513.jpg)
![Neutralization IL-1 RI Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL-1_RI_AF771_Block_Neutralize_8962.jpg)
![Neutralization IL-1 RI Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL-1_RI_AF269_Block_Neutralize_8838.jpg)
![Immunocytochemistry/ Immunofluorescence IL-1 RI Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af269_human-il-1-ri-affinity-purified-polyclonal-ab-8220249505956.jpg)
![Immunocytochemistry/ Immunofluorescence IL-1 RI Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af269_human-il-1-ri-affinity-purified-polyclonal-ab-44202415425024.jpg)
![ELISA IL-1 RI Antibody [Unconjugated]](http://images.novusbio.com/images/af269_human-il-1-ri-affinity-purified-polyclonal-ab-elisa-639201852917905156.jpg)
![Binding Activity IL-1 RI [Biotin]](http://images.novusbio.com/images/avi11085_recombinant-human-il-1-ri-fc-chimera-avi-tag-protein-cf-binding-activity-111020248335.jpg)
![SDS-Page IL-1 RI [Biotin]](http://images.novusbio.com/fullsize/avi11085_recombinant-human-il-1-ri-fc-chimera-avi-tag-protein-cf-sds-page-1110202484536..jpg)
