| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89527-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6348-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6348-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58728-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97789-50ul | |||
![Western Blot: TOP2B Antibody [NBP1-89527] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NBP1-89527-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TOP2B Antibody [NBP1-89527] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP1-89527] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89527-img0011.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human pancreas shows weak to moderate nuclear positivity in islets of Langerhans and exocrine glandular cells.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP1-89527-img0020.jpg)
![Western Blot: TOP2B Antibody [NBP1-89527] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NBP1-89527-img0012.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP1-89527-img0017.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human gastrointestinal shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP1-89527-img0018.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human kidney shows moderate nuclear positivity in cells in glomeruli and tubules.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP1-89527-img0019.jpg)
![Simple Western: TOP2B Antibody [NBP1-89527] - Simple Western lane view shows a specific band for TOP2B in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Simple-Western-NBP1-89527-img0007.jpg)
![Simple Western: TOP2B Antibody [NBP1-89527] - Electropherogram image(s) of corresponding Simple Western lane view. TOP2B antibody was used at 1:100 dilution on RT-4 lysate(s).](http://images.novusbio.com/fullsize/TOP2B-Antibody-Simple-Western-NBP1-89527-img0008.jpg)
![Western Blot: TOP2B Antibody [NB100-40842] - Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Topo II Beta antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence withan exposure time of 30 seconds](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NB100-40842-img0010.jpg "Western Blot: TOP2B Antibody [NB100-40842] - Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Topo II Beta antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence withan exposure time of 30 seconds")
![Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbitanti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NB100-40842-img0009.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TOP2B Antibody [NB100-40842] - Detection of Human Topo II Beta on HeLa whole cell lysate using NB100-40842. Topo II Beta was also immunoprecipitated by another rabbit anti-Topo II Beta antibody and NB100-40841.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NB100-40842-img0007.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NB100-40842-img0008.jpg)
![Western Blot: TOP2B Antibody [NBP2-20684] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Topoisomerase II beta antibody [C3], C-term diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NBP2-20684-img0007.jpg "Western Blot: TOP2B Antibody [NBP2-20684] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Topoisomerase II beta antibody [C3], C-term diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP2-20684] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20684-img0012.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Mouse brain. Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP2-20684-img0011.jpg)
![Western Blot: TOP2B Antibody [NBP2-20684] - Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/Topo-II-beta-Antibody-Western-Blot-NBP2-20684-img0001.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Ovarian cancer, using Topoisomerase II beta antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Topo-II-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-20684-img0002.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Mouse brain. Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP2-20684-img0010.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Mouse testis. Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP2-20684-img0009.jpg)
![Immunoprecipitation: TOP2B Antibody [NBP2-20684] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 ug of Topoisomerase II beta antibody 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunoprecipitation-NBP2-20684-img0003.jpg)
![SDS-Page: Recombinant Human TOP2B Protein [H00007155-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-TOP2B-Protein-SDS-Page-H00007155-Q01-img0002.jpg "SDS-Page: Recombinant Human TOP2B Protein [H00007155-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: TOP2B Antibody [NBP2-58728] - Analysis using Anti-TOP2B antibody NBP2-58728 (A) shows similar pattern to independent antibody NBP1-89527 (B).](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NBP2-58728-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: TOP2B Antibody [NBP2-58728] - Analysis using Anti-TOP2B antibody NBP2-58728 (A) shows similar pattern to independent antibody NBP1-89527 (B).")
![Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP2-97789] - Immunofluorescence staining of TOP2B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TOP2B polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97789-img0001.jpg "Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP2-97789] - Immunofluorescence staining of TOP2B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TOP2B polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.")
![ELISA: Human TOP2B - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38673] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38673_human-top2b-ready-to-use-elisa-kit-colorimetric-211020241528258.png "ELISA: Human TOP2B - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38673] - Standard Curve Reference")
![ELISA: Human TOP2B ELISA Kit (Colorimetric) [NBP3-38674] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38674_human-top2b-elisa-kit-colorimetric-2110202415384027.png "ELISA: Human TOP2B ELISA Kit (Colorimetric) [NBP3-38674] - Standard Curve Reference")