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![ELISA: SARS-CoV-2 Spike Antibody (CR3022) - Azide and BSA Free [NBP2-90980] - ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using NBP2-90980; from 370 ng/ml for NBP3-11813 and from 10000 ng/ml for NBP3-11776. Human IgM and human IgA were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/nbp3-11813_human-monoclonal-sars-cov-2-spike-antibody-cr3022-7420252228560.png "ELISA: SARS-CoV-2 Spike Antibody (CR3022) - Azide and BSA Free [NBP2-90980] - ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using NBP2-90980; from 370 ng/ml for NBP3-11813 and from 10000 ng/ml for NBP3-11776. Human IgM and human IgA were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.")
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-12852] - SARS-Cov-2 Spike 156-157EF Antibodies Specifically Do Not Recognize Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike 1566-157EF antibody, NBP3-12852, 1 ug/mL,, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-12852-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody [NBP3-12852] - SARS-Cov-2 Spike 156-157EF Antibodies Specifically Do Not Recognize Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike 1566-157EF antibody, NBP3-12852, 1 ug/mL,, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.")
![Western Blot: SARS-CoV-2 Spike Antibody [NBP3-12852] - WB Validation of of Spike 156-157EF Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Spike 156-157EF antibody, NBP3-12852, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. SARS-CoV-2 Spike 156-157EF antibody (NBP3-12852) detects spike S1 protein of WT, but not Delta variant.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP3-12852-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike Antibody [NBP3-07035] - Immunofluorescence Validation of SARS-CoV-2 Spike in HeLa cells. Immunofluorescence analysis of HeLa cells, untreated (right) and SARS-CoV-2 Spike plasmid transfected (left) using SARS-CoV-2 Spike antibody (NBP3-07035) at 1:100 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-07035-img0001.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike Antibody [NBP3-07035] - Immunofluorescence Validation of SARS-CoV-2 Spike in HeLa cells. Immunofluorescence analysis of HeLa cells, untreated (right) and SARS-CoV-2 Spike plasmid transfected (left) using SARS-CoV-2 Spike antibody (NBP3-07035) at 1:100 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: SARS-CoV-2 Spike RBD Antibody (2165) [NBP3-07066] - SARS-CoV-2 Spike RBD Antibody (clone 2165) IHC data using infected cell pellets.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-2165-Immunohistochemistry-NBP3-07066-img0003.jpg "Immunohistochemistry: SARS-CoV-2 Spike RBD Antibody (2165) [NBP3-07066] - SARS-CoV-2 Spike RBD Antibody (clone 2165) IHC data using infected cell pellets.")
![Neutralization: SARS-CoV-2 Spike RBD Antibody (2165) [NBP3-07066] - SARS-CoV-2 Spike RBD Antibody to the Receptor Binding Domain (RBD) (Clone 2165). Antibody binds to RBD and blocks binding to the ACE2 receptor on coated plates using a neutralization ELISA assay.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-2165-Neutralization-NBP3-07066-img0002.jpg)
![Neutralization: SARS-CoV-2 Spike RBD Antibody (2165) [NBP3-07066] - Coating: Purified Recombinant SARS-CoV-2 Spike RBD, concentration of 1 ul/well, 100 ul/well overnight 2-8C. Detection: SARS-CoV-2 Spike RBD (Clone 2165) conjugated to HRP was serially diluted starting at 25 ng/ml down to 0.23 ng/ml, 100 ul/well for 1 hour at 37C. Substrate: TMB, 100 ul/well for 15 min. at room temperature followed by 450 nm stop solution, 50ul/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-2165-Neutralization-NBP3-07066-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) - Azide and BSA Free [NBP2-90980] - ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using NBP2-90980; from 370 ng/ml for NBP3-11813 and from 10000 ng/ml for NBP3-11776. Human IgM and human IgA were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/nbp3-11776_human-monoclonal-sars-cov-2-spike-antibody-cr3022-7420252228564.png "ELISA: SARS-CoV-2 Spike Antibody (CR3022) - Azide and BSA Free [NBP2-90980] - ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using NBP2-90980; from 370 ng/ml for NBP3-11813 and from 10000 ng/ml for NBP3-11776. Human IgM and human IgA were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.")
![ELISA: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12851] - SARS-Cov-2 Spike L452R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12851, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Delta-Variant--B.1.617.2--India-ELISA-NBP3-12851-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12851] - SARS-Cov-2 Spike L452R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12851, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.")
![Western Blot: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12851] - WB Validation of of Delta Variant Spike Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12851, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. SARS-CoV-2 Delta Variant Spike antibody (NBP3-12851) specifically detects Delta variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Delta-Variant--B.1.617.2--India-Western-Blot-NBP3-12851-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody (B005N) - (ECD) [NBP3-05722] - Western Blot Validation with Recombinant Protein. Loading: 1ug of recombinant protein per lane. Lane 1: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike, NBP3-05722, 1:4000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-B005N-ECD-Western-Blot-NBP3-05722-img0002.jpg "Western Blot: SARS-CoV-2 Spike Antibody (B005N) - (ECD) [NBP3-05722] - Western Blot Validation with Recombinant Protein. Loading: 1ug of recombinant protein per lane. Lane 1: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike, NBP3-05722, 1:4000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.")
![ELISA: SARS-CoV-2 Spike Antibody (B005N) - (ECD) [NBP3-05722] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-RBD Recombinant Protein, and column 2: SARS-CoV-2 Spike-ECD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-B005N-ECD-ELISA-NBP3-05722-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12853] - SARS-Cov-2 Spike 156-157EFdel ( Delta Variant ) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12853, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Delta-Variant--B.1.617.2--India-ELISA-NBP3-12853-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12853] - SARS-Cov-2 Spike 156-157EFdel ( Delta Variant ) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12853, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.")
![Western Blot: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12853] - WB Validation of of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12853, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. SARS-CoV-2 Delta Variant Spike antibody (NBP3-12853) specifically detects Delta variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Delta-Variant--B.1.617.2--India-Western-Blot-NBP3-12853-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike RBD Antibody (B001Y) - (RBD) [NBP3-05725] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B001Y-RBD-Western-Blot-NBP3-05725-img0001.jpg "Western Blot: SARS-CoV-2 Spike RBD Antibody (B001Y) - (RBD) [NBP3-05725] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (B001Y) - (RBD) [NBP3-05725] - ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike RBD Antibody, NBP3-05725. Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution. Develop: 15min, 100 uL/well. Stop: Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B001Y-RBD-ELISA-NBP3-05725-img0002.jpg)