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![ELISA: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12853] - SARS-Cov-2 Spike 156-157EFdel ( Delta Variant ) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12853, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Delta-Variant--B.1.617.2--India-ELISA-NBP3-12853-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12853] - SARS-Cov-2 Spike 156-157EFdel ( Delta Variant ) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12853, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.")
![Western Blot: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12853] - WB Validation of of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12853, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. SARS-CoV-2 Delta Variant Spike antibody (NBP3-12853) specifically detects Delta variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Delta-Variant--B.1.617.2--India-Western-Blot-NBP3-12853-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike S2 Antibody [NBP3-05700] - Validation with SARS-CoV-2 Spike S2 ECD Recombinant Protein Loading: 30 ng per lane of SARS-CoV-2 Spike S2 ECD recombinant protein, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-S2-Antibody-Western-Blot-NBP3-05700-img0001.jpg "Western Blot: SARS-CoV-2 Spike S2 Antibody [NBP3-05700] - Validation with SARS-CoV-2 Spike S2 ECD Recombinant Protein Loading: 30 ng per lane of SARS-CoV-2 Spike S2 ECD recombinant protein, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.")
![ELISA: SARS-CoV-2 Spike S2 Antibody - BSA Free [NBP3-05700] - Figure 2 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, 9181. A direct ELISA was performed using immunogen peptide (P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.](http://images.novusbio.com/fullsize/nbp3-05700_rabbit-polyclonal-sars-cov-2-spike-s2-antibody-2711202420414569.jpg)
![ELISA: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12851] - SARS-Cov-2 Spike L452R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12851, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Delta-Variant--B.1.617.2--India-ELISA-NBP3-12851-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12851] - SARS-Cov-2 Spike L452R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12851, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.")
![Western Blot: SARS-CoV-2 Spike Antibody - Delta Variant, B.1.617.2, India [NBP3-12851] - WB Validation of of Delta Variant Spike Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, NBP3-12851, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. SARS-CoV-2 Delta Variant Spike antibody (NBP3-12851) specifically detects Delta variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Delta-Variant--B.1.617.2--India-Western-Blot-NBP3-12851-img0002.jpg)
![ELISA: Human HBsAg ELISA Kit (Colorimetric) [NBP3-11804] - Samples were spiked with high concentrations of Human HBsAg and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-11804_human-hbsag-elisa-kit-colorimetric-132202416175645.jpg "ELISA: Human HBsAg ELISA Kit (Colorimetric) [NBP3-11804] - Samples were spiked with high concentrations of Human HBsAg and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human HBsAg ELISA Kit (Colorimetric) [NBP3-11804] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-11804_human-hbsag-elisa-kit-colorimetric-4320249133282.jpg)
![Western Blot: Human Coronavirus Spike Protein Antibody (06) [NBP3-14654] - Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-His SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-06-Western-Blot-NBP3-14654-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (06) [NBP3-14654] - Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-His SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![Western Blot: Human Coronavirus Spike Protein Antibody (23) [NBP3-14657] - Anti-Coronavirus Spike Protein mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His Lane 12: HCoV-OC43 Spike S1+S2 ECD-His Secondary: Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-23-Western-Blot-NBP3-14657-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (23) [NBP3-14657] - Anti-Coronavirus Spike Protein mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His Lane 12: HCoV-OC43 Spike S1+S2 ECD-His Secondary: Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (T5P7-G10) - VHH [NBP3-12845] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12845) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12845 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-T5P7-G10-VHH-ELISA-NBP3-12845-img0001.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (T5P7-G10) - VHH [NBP3-12845] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12845) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12845 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (T5P7-G10) - VHH - Azide and BSA Free [NBP3-12845] - Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants. Antibodies: SARS-CoV-2 Spike RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1 ug/mL and each of the 6 anti-SARS-CoV-2 Spike RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SD9439, , and SD9503 bind spike proteins of omicron variant, but not delta variant; SD9511 and SD9437 bind spike proteins of delta variant, but not omicron variant; SD9507 binds spike proteins of delta and omicron variants.](http://images.novusbio.com/fullsize/nbp3-12845_llama-monoclonal-sars-cov-2-spike-rbd-antibody-t5p7-g10-vhh-2711202419432249.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (RBD-2B9) - VHH [NBP3-12843] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12843) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike S1 RBD Antibody, NBP3-12843 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-RBD-2B9-VHH-ELISA-NBP3-12843-img0001.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (RBD-2B9) - VHH [NBP3-12843] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12843) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike S1 RBD Antibody, NBP3-12843 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (RBD-2B9) - VHH - Azide and BSA Free [NBP3-12843] - Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants. Antibodies: SARS-CoV-2 Spike RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1 ug/mL and each of the 6 anti-SARS-CoV-2 Spike RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SD9439, SD9441, and SD9503 bind spike proteins of omicron variant, but not delta variant; SD9511 and bind spike proteins of delta variant, but not omicron variant; SD9507 binds spike proteins of delta and omicron variants.](http://images.novusbio.com/fullsize/nbp3-12843_llama-monoclonal-sars-cov-2-spike-rbd-antibody-rbd-2b9-vhh-271120241932665.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (T5P4-A12) - VHH [NBP3-12844] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12844) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12844 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-T5P4-A12-VHH-ELISA-NBP3-12844-img0001.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (T5P4-A12) - VHH [NBP3-12844] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12844) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12844 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (T5P4-A12) - VHH - Azide and BSA Free [NBP3-12844] - Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants. Antibodies: SARS-CoV-2 Spike RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1 ug/mL and each of the 6 anti-SARS-CoV-2 Spike RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. , SD9441, and SD9503 bind spike proteins of omicron variant, but not delta variant; SD9511 and SD9437 bind spike proteins of delta variant, but not omicron variant; SD9507 binds spike proteins of delta and omicron variants.](http://images.novusbio.com/fullsize/nbp3-12844_llama-monoclonal-sars-cov-2-spike-rbd-antibody-t5p4-a12-vhh-27112024202296.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (T5P8-F9) - VHH [NBP3-12846] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12846) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12846 can detect spike RBD protein at 10 ng/mL and can weakly detect spike S1 protein at 200 ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-T5P8-F9-VHH-ELISA-NBP3-12846-img0001.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (T5P8-F9) - VHH [NBP3-12846] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12846) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12846 can detect spike RBD protein at 10 ng/mL and can weakly detect spike S1 protein at 200 ng/mL.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (T5P8-F9) - VHH - Azide and BSA Free [NBP3-12846] - Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants. Antibodies: SARS-CoV-2 Spike RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1 ug/mL and each of the 6 anti-SARS-CoV-2 Spike RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SD9439, SD9441, and bind spike proteins of omicron variant, but not delta variant; SD9511 and SD9437 bind spike proteins of delta variant, but not omicron variant; SD9507 binds spike proteins of delta and omicron variants.](http://images.novusbio.com/fullsize/nbp3-12846_llama-monoclonal-sars-cov-2-spike-rbd-antibody-t5p8-f9-vhh-271120242092083.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (T3P1-C8) - VHH [NBP3-12849] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12849) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12849 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-T3P1-C8-VHH-ELISA-NBP3-12849-img0001.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (T3P1-C8) - VHH [NBP3-12849] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12849) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12849 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (T3P1-C8) - VHH - Azide and BSA Free [NBP3-12849] - Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants. Antibodies: SARS-CoV-2 Spike RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1 ug/mL and each of the 6 anti-SARS-CoV-2 Spike RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SD9439, SD9441, and SD9503 bind spike proteins of omicron variant, but not delta variant; and SD9437 bind spike proteins of delta variant, but not omicron variant; SD9507 binds spike proteins of delta and omicron variants.](http://images.novusbio.com/fullsize/nbp3-12849_llama-monoclonal-sars-cov-2-spike-rbd-antibody-t3p1-c8-vhh-2711202420115567.jpg)